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dr.antoine sayegh researches Home
The newest dr. Antoine Sayegh declarations on the cancer treatments and for the aids also : http://sayeghresearches.wetpaint.com/ My great dear: please contact me on these E-mails in the same time to confirm your sending of your e-mails to me E-mails: antsay@aloola.sy dr.antoine7sayegh@yahoo.com antsay@37.com dr.antsay@gmail.com the websites : http://www.facebook.com/people/Antoine-Sayegh/100002063614016 http://www.facebook.com/pages/DrAntoine-Sayegh/271293289555800 http://sayeghresearches.wetpaint.com/ http://antsay.webs.com/ http://www.facebook.com/pages/Syria-Blue-Crescent/181980491876373
with the best regards from the dr. Antoine Sayegh
http://antsay.webs.com/apps/photos http://sayeghresearches.wetpaint.com/photo/11223556/internatinal+certificate http://www.iainternalmeds.com/certificate.6128612.html
The newest dr. Antoine Sayegh declarations on the cancer treatments and aids also :
I call all the cancer research centers of the world to concentrate on my new suggestions in their trials on cancer and on aids also : We must use the combinations from many drugs which act on the ( Myristate , farensyl ,glucosylphosphatidylinositol ) in the same time in the protection from cancers which promise us for the new the treatments for the aids and the cancer also. The membrane-attached proteins divided into three groups: 1.group A : bound to the cytosolic face of the plasma membrane by myristate as v-Src is a kind of non-receptor protein tyrosine kinase involved in cell signaling: 1.we must get more benefits from the properties of the Dihydropyridine-type calcium-channel antagonists (DTCCA) which plays an important roles in many diseases , because they decrease vasospastic propensity. These drugs are suspected to have anti-oxidant properties in other diseases as in the systemic sclerosis . that nifedipine and nicardipine decrease circulating markers of oxidative stress damage in patients.. the effects of nifedipine on O2•- release from human monocytes, on protein phosphorylation with phorbol myristate acetate (PMA) as stimulator and on protein kinase C (PKC) activity, This beneficial property of nifedipine seems to be mediated by its cellular action and by the inhibition of PKC activity,so we can use this drugs against the diseases which associate with the oxidative harms. ( Paris V University, Cochin Hospital, Cochin Institute,france) 2.we must get more benefits also from the relations from the Phorbol 12-Myristate 13-acetate (PMA) and angiotensin II (Ang II)which increased both the percentage of scavenger receptors Scr-positive cells and the Scr mean fluorescence intensity. PMA and Ang II also increased Scr mRNA and promoter activity in a time- and dose-responsive manner. Protein kinase C and calmodulin transduction pathways were involved in Scr up-regulation induced by PMA and Ang II. Additionally, a serine/threonine kinase was involved in PMA stimulation. Functional analysis showed that both AP-1 and ets motifs were specific response elements to PMA stimulation in HMCLs ,so the drugs which change the relations between the PMA and the ANGII which inhibits the actions of the ANGII can breaks down the transduction pathways (Center for Nephrology, Royal Free and University College Medical School, Royal Free Campus, London, England, United Kingdom) 2. group B:bound to the cytosolic face of the plasma membrane by farnesyl as Ras protein which also plays a key role in cell signaling: From my participation with Webinar on the antitumor effects of the biophosphonate in breast cancer 1. The gene on the chromosome 1 and the location q22 encodes an enzyme that catalyzes the production of geranyl pyrophosphate and farnesyl pyrophosphate from isopentenyl pyrophosphate and dimethylallyl pyrophosphate. The resulting product, farnesyl pyrophosphate, a substrate for protein farnesylation and geranylgeranylation, Drugs Zoledronic acid is an amino-bisphosphonate that inhibit this enzyme FPP [farnesyl diphosphate] synthase prevent the post-translational modifications of small GTPases and have been used to treat diseases related to bone resorption. Multiple pseudogenes have been found on chromosomes 1, 7, 14, 15, 21 and X. Multiple transcript variants encoding different isoforms have been found for this gene .so we can can the Bisphosphonates as clodronate and zoledronic acid to inhibit also the other isoforms . 2.the second point to avoid the the harm effects on the normal cells from the uses of the Bisphosphonates as clodronate and zoledronic acid , we can use the antibodies against the FPP [farnesyl diphosphate] synthase as in my theoretic researches for temporary periods instead of the biophosphonates Please visit the all researches I and all researches II on my website http://sayeghresearches.wetpaint.com/ http://sayeghresearches.wetpaint.com/forum 3.from the effects of the uses of the biophosphonates on the relation the FPP [farnesyl diphosphate] synthase as a molecular partner for p13(II) and G4 accessory proteins which opens new prospects for treatment of retrovirus-induced leukemia HTLV1 p13(II) not on cancer but on the retroviruses also 4, the HMG-CoA reductase inhibitors (statins) as an anticancer drugs: from the international researches we found : ( University of Mainz, Germany ) Apart from their lipid lowering activity, HMG-CoA reductase inhibitors (statins) impair numerous cellular functions associated with metastasis, e.g. gene expression, angiogenesis, cell adhesion, cell motility and invasiveness. Furthermore, statins have impact on apoptotic cell death and modulate cellular susceptibility to cell killing by anticancer drugs and ionizing radiation. Part of the effects provoked by statins are due to the inhibition of the prenylation of low molecular weight GTPases, in particular Ras and Rho, which play key roles in signaling evoked by stimulation of cell surface receptors. C-terminal lipid modification of Ras/Rho GTPases is essential for their correct intracellular localization and function. By depletion of the cellular pool of isoprene precursor molecules, statins reduce the level of membrane-bound active Ras/Rho proteins, thereby impairing corresponding functions. Since broad clinical experience already exists for statins, their incorporation into established tumor-therapeutic regimens would be realizable in a rather short period of time. Here, data available at present arguing for the usefulness of statins in anticancer therapy are summarized and discussed. 3. group C: bound to the extracelluar face of the plasma membrane by glycosylphosphatidylinositol;(dr. Antoine Sayegh clinic Aleppo ,Syria) 1.the amlodipine acts as Calcium channels blockers (CCB) which inhibits for the CA++ to enter intracellular from many mechanisms by the blocking the voltage channels dependent for the CA++ or from the acting on the receptor channels dependent for CA++ ,and from the other actions of the CCB on the relations between the CA++ and the IP3 which plays an important roles on its formation from the PIP2 to IP3 + DAG and on its receptors ( ip3)also from the effects of the CA++ on the ubiquitin proteasome pathway ,so from the last roles of the CCB which inhibits the actions of the CA++ on the relations between the CA++ and the IP3 and on the also IP3 receptors So we can we use the CCB for the treatment for the cancers ,because the IP3 and the CA++ with the relation with the calcineurin and the active NFAT help for the genes transcription , so ,we must study the patients which treated from the CCB and we must evaluate the ratios of the cancers in these groups of the patients ( the ratios in this group very very low ) ,also from the roles of the multiple kinase as the(calcium/calmodulin-dependent protein kinase (CaMK),with the other proteinkinase as protein kinase D (PKD), microtubule affinity-regulating kinase (MARK), salt-inducible kinase (SIK), checkpoint kinase-1 (CHK1) and other kinases) mediate specific phosphorylation of human histone deacetylase-4 (HDAC4) on three 14-3-3-binding sites. Myosin phosphatase-targeting subunit-1 (MYPT1)–protein phosphatase-1 (PP1) and PP2A can also act on these sites. The association of 14-3-3 proteins with HDAC4 retains it in the cytoplasm and prevents its interaction with transcription factors such as myocyte enhancer factor-2 (MEF2), thereby releasing these transcription factors for transcriptional activation. So when we inhibits the (calcium/calmodulin-dependent protein kinase (CaMK)which acts with other mechanisms in the signaling of the CA++ for the translations and for the transductions or transcription on the genes by the STAT or SMAD4 pathways
2. we use the angiotensin converting enzyme inhibitors (ACEI) with the CCB so to get the most important synergistic effects from their combination , and because the ACEI inhibits the actions of the Ang II ,and from the relation from the Ang II on the ATII receptor and on the ATI receptor especially also ( angiotensin I receptor) which excite the formation of the IP3 also from the PLC which acts to form the IP3 and the DAG from the PIP2,so the IP3+ the CA++ and the DAG activates the PKC which play as the vasoconstrictive roles in the hypertension ( HT) , so when we use the combination from the ACEI +CCB we get the best effects on the HT from the vasodilatation roles and from the indirect effects on the IP3 formation, so we can use the ACEI + CCB in the treatment of the cancer in the futures also .
3.the most important notices for the treatments for the cancer from two new ways : a. the neprilysin path ways :(from the mechanism of degradation ) which convert the GTP to c GMP with the CA++ which act as vasodilatation:
1.the first way: the neprilysin(nep) activate( degradation )the ANP which convert the GTP to c GMP .
2.the second way :the nep acts ( degradation) on the BK to BK2and the BK2 NOS convert the L Arg to NO (nitrous oxide ) which also convert the GTP to c GMP with the CA++ to dilate the vessels. So the drugs which activate the neprilysin pathways cause the vasodilatation with the important roles of the NO on the genes
2. the effects of the :
a. angiotensin converting enzyme inhibitors (ACEI)which decrease the breakdown of the bradykinin.
b. the calcium channels blockers (CCB ) which increase the synthesis of the bradykinin . the bradykinin which binds to the b2 kinin receptors which acts as:
1. on the coronary epithelium activate the intracellular NO synthase and to release the endothelium derived NO.
2.on the myocardium : diffuses of the NO in the myocytes and regulates the mitochondrial respiration .
But the NO in the tissues plays an important roles in the cancers because the macrophages :these cells activated by the cytokines or by the TNF which produces the nitric oxide ( NO ) which kills the organisms ( for the aids viruses also) and the malignant cells ( cancers )and the macrophages which aid and which did not aid also by the antibodies .
So the most important uses of the combination from the ACEI with the CCB in the future to treat the different cancers and for the different organisms like the aids also .
4. can we use the combination also for the treatment for HT as before between the ACEI plus angiotensin II receptor blockers( ARB) to get more synergistic effects when we face the genetic mutations on the ANG gene, Ang I and Ang I receptor genes ,or on the ADDI ( adducin gene )??? . Please answer me as fast as you can The newest relations between the Calcium channels blockers (CCB) and the angiotensin converting enzyme inhibitors (ACEI) with the transcription factor nuclear factor E2-related factor 2 (Nrf2) directly or indirectly as anti oxidant or anti cancer The proofs : 1. Nrf2 is a protein messenger contained in every cell of the body that sends information to the cell’s DNA, When this protein messenger called Nrf2 is activated, it enters the nucleus of every cell and it turns on several hundred of the DNA genes. And the genes that it turns on, or turns up, are known collectively as “survival genes.” These genes enable cells to survive in the face of several different kinds of stress, especially oxidative stress which is due to the over production of free radicals and other oxidants. many researches confirm my declaration of the link : WikiGenes - NFE2L2 - nuclear factor (erythroid-derived 2)-like 2.mht 2. Flunarizine induces Nrf2-mediated transcriptional activation of heme oxygenase-1 in protection of auditory cells from cisplatin H-S So, H-J Kim, J-H Lee, J-H Lee, S-Y Park, C Park, Y-H Kim, J-K Kim, K-M Lee, K-S Kim, S-Y Chung, W-C Jang, S-K Moon, H-T Chung and R-K ParPretreatment with flunarizine predominantly induced the transcriptional expression of HO-1 among other Nrf2-regulated genes in cisplatin-treated cells. (a) Cells were treated with 20 M cisplatin for the indicated periods in the presence or absence of 10 M flunarizine. Then, total RNA was isolated by RNAzol and cDNA was synthesized by reverse transcriptase. The Nrf2-related genes, including HO-1, NQO1, GCLC, GCLM, GST-1, and GSTA4, were amplified with specific primer sets, respectively. Also, cells were treated with 20 M cisplatin, 10 M flunaizine and various doses of SnPPIX for 36 h and used to measure the cell viability (b), to determine the enzymatic activity of HO-1 at 20 h (c), or to test the expression level of HO-1 protein by Western blotting (d). # P<0.01, ** P<0.01 by one-way ANOVA, compared with only cisplatin treated group (#) or flunarizine/cisplatin-cotreated group (*, **). (e) To examine the effect of calcium channel blockers on the HO-1 induction, cells were treated with various calcium channel blockers for 24 h. Cell lysates were separated on 12% SDS-PAGE to probe for HO-1 and -actin by Western blot. Nicardipine, 10 M; nifedipine, 12.5 M; ditiazem, 10 M; pimozide, 2.5 M 3. Nitric oxide activation of Keap1/Nrf2 signaling in human colon carcinoma cells Chun-Qi Li Min Young Kim Luiz C. Godoy Apinya Thiantanawat Laura J. Trudel Gerald N. Wogan The transcription factor NF-E2-related nuclear factor 2 (Nrf2) regulates expression of genes that protect cells from oxidative damage. Here, we characterized nitric oxide (•NO)-induced Nrf2–Kelch-like ECH-associated protein 1 (Keap1) signaling and its role in counteracting •NO-induced apoptosis of human colon cancer HCT116 cells. Nrf2 was localized in the cytoplasm in control cells; •NO triggered its rapid nuclear accumulation, transcriptional activation, and up-regulation of HO-1, NQO1, and GCL, but not GST A4 and P1 subunits. Nrf2 accumulation in the nucleus was also associated with enhanced transcription and posttranscriptional modifications. (S)-nitrosation of Keap1 may contribute to nuclear accumulation of Nrf2 by facilitating its dissociation from Keap1, thus initiating •NO-mediated Nrf2–Keap1 signaling. •NO-mediated induction of ARE-dependent genes occurred well before apoptosis, as judged by caspase 3 activation. Collectively, these results show that the Nrf2–Keap1 signaling pathway mediates protective cellular responses to mitigate •NO-induced damage and may contribute to the relative resistance of HCT116 to •NO-induced cytotoxicity. 4.the researches from iHOP - Information Hyperlinked over Proteins [ NPPA ].mht a. To verify the hypothesis that the angiotensin converting enzyme (ACE) level may affect the metabolism of circulating atrial natriuretic factor (ANF), the acute and chronic effects of benazepril on plasma ANF levels were studied in hypertensive patients under basal conditions and in response to acute volume expansion b. Atrial natriuretic peptide (ANP ), C-type natriuretic peptide, and C-ANP-(4-23), a ligand for the natriuretic peptide clearance receptor, equipotently inhibited production of VEGF by as much as 88% and inhibited ET- or hypoxia-stimulated VEGF transcription c. Atrial natriuretic peptide(ANP) has been shown to reduce tumor necrosis factor-alpha (TNF-alpha)-induced activation of endothelial cells via inhibition of p38 mitogen-activated protein kinase (MAPK ) and nuclear factor (NF)-kappaB pathways. d. Atrial natriuretic factor 1-28 (ANF) and C-type natriuretic factor-22 (CNP) reduced angiotensin II (Ang II)- and platelet-derived growth factor-stimulated PAI-1 mRNA expression in rat aortic smooth muscle cells by 50% to 70%, with corresponding reductions in PAI-1 protein release e. Arial natriuretic factor ANP evoked production of superoxide was found to activate c-Jun N-terminal kinase (JNK). f. Atrial natriuretic peptide (ANP)-C receptor activation has been shown to inhibit adenylyl cyclase (AC) activity as well as to stimulate phospholipase C (PLC) signaling pathways. 5.researches from : WikiGenes - Evolutionary Knowledge.mht 1. NFE2L2 - nuclear factor (erythroid-derived 2)-like 2 Gene: induction via Nrf2 activation in hepatoma cells. Early effects( only 1 h of exposure) of Disease relevance of NFE2L2 Human prx1 gene is a target of Nrf2 and is up- regulated by hypoxia/ reoxygenation: implication to tumor biology. Neuroblastoma cells were stably transfected with DN- Nrf2, which/ Synonyms: NRF2 2. MAPKAP1 - mitogen-activated protein kinase... Gene: Physical interactions of MAPKAP1 Transfection of PC12 cells with dominant- negative Nrf2 abolished the SIN- 1- induced increase in Nrf2- ARE binding and subsequent upregulation of HO- 1 expression, leading neuroblastoma cells( TAM- 67 cells) to apoptosis induced by the nitric oxide( NO) 3. Hmox1 - heme oxygenase (decycling) 1 Gene: stress induced the nuclear import of Nrf2 and the binding of Nrf2 to the HO- 1 antioxidant and inducible nitric oxide synthase( iNOS) expression in the aortas of ovariectomized rats, and the regulatory heme oxygenase( HO) and calcium- dependent nitric oxide synthase( cNOS) activities were increased
With the best regards from the dr. Antoine Sayegh The new dr. Antoine Sayegh methods for treatments from the stem cells: http://sayeghresearches.wetpaint.com/page/The+new+dr.+Antoine+Sayegh+methods+for+treatments+from+the+stem+cells%3A http://antsay.webs.com/ the aims for the treatments : 1.to use the new methods for the treatments of the chronic and for the fatal diseases also. 2.for the new hopes in the treatments for the different cancers from the killer cells( K cells ,LAK cells ), in the bone marrow suppressions ,and for the aids also from the new created of the CD4 cells . 3.to replace the harmed cells in the different tissues from the infective diseases . 4.to open the new treatments in the future for the hereditary diseases. The sources of the stem cells 1. from placenta. 2. from the adults bone marrow and from the adipose tissues . 3. from the cloning from the somatic cell nucleus transfer to the unucleated ovum as a new totipotent cells which form the blastocyte which gave us the new stem cells . 4.the methods of the dr. James Thompson : to get the pluripotent cells from the blostocyte. 5. the methods of the dr. John Gearhart :to get the stem cells from the embryonic germ cells . The methods which isolate and purify human of the different stem cells from the bone marrow or from the adipose tissues or from other sources, which use different materials for the isolation and for the culture for these cells in the different steps 1. for the processing of the bone marrow mononuclear (adipose , placental) cells. 2. for the magnetic activated cell sorting and for the fluorescence activated cells sorting also. 3.for the culture of the human monoclonal bone marrow cells. These methods use many harmful materials for the stem cells which weak its metabolic vitalities and can change its normal properties or qualities to shortening its ages and help for the deviation toward the oncogene positions ( as carcinogenic cells in the recipient tissues ) . The new dr. Antoine Sayegh methods as below: The first step :the preparing of the isolated and purified stem cells from the bone marrow: a. we take the bone marrow from the iliac crest from the human being . b. we divide the specimen into two parts : 1. we inject the first part in the blood of the animal to form against every component the antibodies the IGM and the IGG to use them later . 2. we incubate the second part (of the bone marrow ) with the animal antibodies which we got it from the first step ( the serum of the animal ) ,and in the same times we incubate the second part with the anti anti bodies the hyper variable part prepared against the anti bodies for the target cells which we need for the isolation like the CD34 or the CD38( from the known isolated cells ) to destroy all the unneeded cells and to protect the target cells . *****The preparation of the hyper variable part of the anti –antibodies from the ( known isolated target cells like CD34 ,CD38, or for ES embryonic stem cells): a. we get the antibodies against the markers proteins ( against the known makers proteins of the target cell from any person as the SSEA-3 (Stage-specific embryonic antigen) ,SEA-4,TRA-1-60(Tumor rejection antigen-1) ,TRA-1-81 or the markers of the CD34 ,CD38 ) b. we incubate part of these antibodies with the pepsin to get the Fab(ab)2 the binding antigen sites ,and the other part with the papain to get the fab(ab)1 and the fragment c (FC)to get different types of the FAB parts with the different types of the FC also in the same times .. c. we incubate the bone marrow of the patient with the different types of the antibodies residues (held on latex) with the different suitable complements proteins to form heavy immune complexes ,we centrifuge the complexes to get from the layer of the latex the target cells . d. we isolate the target cells from the complexes from the cooling or heating , or from the changes of the ph ,so we get the weakened target cells to use them later to form from them only the anti antibodies. e. we can use instead of the latex ,the red blood cells held on its surfaces the hyper variable part of the antibodies against the different types of the FABs which act as the target antigens to form with the bone marrow of the patients with different FABs residue with complements proteins also the agglutinations forms of the immune response to collect between its nets the weakened target cells . 3. we incubate and culture the residues of the second part to get more of the target cells with its high vital activities while the other cells weakened and destroyed from the different antibodies from the uses of the STAT 3 factor and Oct-4 transcription factor which activate the LIF receptor to activate LIF –STAT 3 Signaling pathway which promotes Embryonic Stem cell self-renewal . . 4. we isolate the residues from the low speeds by the centrifuge to avoid the harms for the target cells to get the pure target cells because when these cells were cultured it gained very huge masses which precipitate it easily to the bottom of the tube. 5. we wash the cultured cells from the saline or from not harmful fluids (buffers) many times to clean it from the other ineffective attached cells. The second step: 1. we inject the patients from the vaccine( under the skin) which composed from the anti antibodies( the hyper variable parts of the antibodies ) against the target cells ( CD34,CD38 ,other cells like Embryonic Stem Cells) before many days of the uses of the stem cells ,to destroy every anti bodies against the target cells from the immune response of the uses of the stem cells.. 2. we inject the patients from the new purified stem cells with the low numbers ( not millions but hundreds thousands ) to decrease the cost( money payments) of the treatments . 3.we help the recipient tissues to receive the new stem cells with the low numbers ( hundreds thousands ) and to elongate its ages and to embrace into the defected tissues as a new normal cells from: the next third step : to confirm the new actions of the injected stem cells : a. by the injection of the angiogenesis factors into the blood of the patients , as FGFs , FGF1( the potent factors for angiogenesis ) and as FGF2 , VEGF factors also and from the TGF beta to help the stem cells to invade the recipient tissues . b. we inject into the blood of the patients also the helper the HGF and the MET which activates the proliferations of the stem cells with the E-cadherine, or from the uses of the ET-1 ( endotheline-1) which help the stem cells to be accepted from the target tissues . so the new treatments from the new methods of the isolated stem cells promise us for the best treatments for the fatal diseases in the future which act near the normal cells mechanisms to re repair the defected ( failed ) tissues or organs . My new E-mails: antsay@aloola.sy dr.antoinesayegh@gmail.com dr.antoine7sayegh@dcemail.com dr.antoine7sayegh@yahoo.com dr.antsay@gmail.com dr.antoinesayegh@mail.com the websites : http//:sayeghresearches.wetpaint.com http//:antsay.webs.com http://www.facebook.com/people/Antoine-Sayegh/100002063614016 with the best regards from the dr. Antoine Sayegh
The newest dr. Antoine Sayegh declarations on the cancer treatments and for the aids also : http://sayeghresearches.wetpaint.com/page/The+newest++dr.+Antoine+Sayegh+declarations+on+the+cancer+treatments+and+for+the+aids+also+%3A My great dear: please contact me on these E-mails in the same time to confirm your sending of your e-mails to me
E-mails: antsay@aloola.sy dr.antoinesayegh@dcemail.com dr.antoine7sayegh@yahoo.com dr.antsay@gmail.com
the websites :
http//:sayeghresearches.wetpaint.com http//:antsay.webs.com
with the best regards from the dr. Antoine Sayegh
The newest dr. Antoine Sayegh declarations on the cancer treatments and aids also :
I call all the cancer research centers of the world to concentrate on my new suggestions in their trials on cancer and on aids also : We must use the combinations from many drugs which act on the ( Myristate , farensyl ,glucosylphosphatidylinositol ) in the same time in the protection from cancers and and which promise us for the new the treatments for the aids and the cancer also. The membrane-attached proteins divided into three groups: 1.group A : bound to the cytosolic face of the plasma membrane by myristate as v-Src is a kind of non-receptor protein tyrosine kinase involved in cell signaling: 1.we must get more benefits from the properties of the Dihydropyridine-type calcium-channel antagonists (DTCCA) which plays an important roles in many diseases , because they decrease vasospastic propensity. These drugs are suspected to have anti-oxidant properties in other diseases as in the systemic sclerosis . that nifedipine and nicardipine decrease circulating markers of oxidative stress damage in patients.. the effects of nifedipine on O2•- release from human monocytes, on protein phosphorylation with phorbol myristate acetate (PMA) as stimulator and on protein kinase C (PKC) activity, This beneficial property of nifedipine seems to be mediated by its cellular action and by the inhibition of PKC activity,so we can use this drugs against the diseases which associate with the oxidative harms. ( Paris V University, Cochin Hospital, Cochin Institute,france) 2.we must get more benefits also from the relations from the Phorbol 12-Myristate 13-acetate (PMA) and angiotensin II (Ang II)which increased both the percentage of scavenger receptors Scr-positive cells and the Scr mean fluorescence intensity. PMA and Ang II also increased Scr mRNA and promoter activity in a time- and dose-responsive manner. Protein kinase C and calmodulin transduction pathways were involved in Scr up-regulation induced by PMA and Ang II. Additionally, a serine/threonine kinase was involved in PMA stimulation. Functional analysis showed that both AP-1 and ets motifs were specific response elements to PMA stimulation in HMCLs ,so the drugs which change the relations between the PMA and the ANGII which inhibits the actions of the ANGII can breaks down the transduction pathways (Center for Nephrology, Royal Free and University College Medical School, Royal Free Campus, London, England, United Kingdom) 2. group B:bound to the cytosolic face of the plasma membrane by farnesyl as Ras protein which also plays a key role in cell signaling: From my participation with Webinar on the antitumor effects of the biophosphonate in breast cancer 1. The gene on the chromosome 1 and the location q22 encodes an enzyme that catalyzes the production of geranyl pyrophosphate and farnesyl pyrophosphate from isopentenyl pyrophosphate and dimethylallyl pyrophosphate. The resulting product, farnesyl pyrophosphate, a substrate for protein farnesylation and geranylgeranylation, Drugs Zoledronic acid is an amino-bisphosphonate that inhibit this enzyme FPP [farnesyl diphosphate] synthase prevent the post-translational modifications of small GTPases and have been used to treat diseases related to bone resorption. Multiple pseudogenes have been found on chromosomes 1, 7, 14, 15, 21 and X. Multiple transcript variants encoding different isoforms have been found for this gene .so we can can the Bisphosphonates as clodronate and zoledronic acid to inhibit also the other isoforms . 2.the second point to avoid the the harm effects on the normal cells from the uses of the Bisphosphonates as clodronate and zoledronic acid , we can use the antibodies against the FPP [farnesyl diphosphate] synthase as in my theoretic researches for temporary periods instead of the biophosphonates Please visit the all researches I and all researches II on my website http://sayeghresearches.wetpaint.com/ http://sayeghresearches.wetpaint.com/forum 3.from the effects of the uses of the biophosphonates on the relation the FPP [farnesyl diphosphate] synthase as a molecular partner for p13(II) and G4 accessory proteins which opens new prospects for treatment of retrovirus-induced leukemia HTLV1 p13(II) not on cancer but on the retroviruses also 4, the HMG-CoA reductase inhibitors (statins) as an anticancer drugs: from the international researches we found : ( University of Mainz, Germany ) Apart from their lipid lowering activity, HMG-CoA reductase inhibitors (statins) impair numerous cellular functions associated with metastasis, e.g. gene expression, angiogenesis, cell adhesion, cell motility and invasiveness. Furthermore, statins have impact on apoptotic cell death and modulate cellular susceptibility to cell killing by anticancer drugs and ionizing radiation. Part of the effects provoked by statins are due to the inhibition of the prenylation of low molecular weight GTPases, in particular Ras and Rho, which play key roles in signaling evoked by stimulation of cell surface receptors. C-terminal lipid modification of Ras/Rho GTPases is essential for their correct intracellular localization and function. By depletion of the cellular pool of isoprene precursor molecules, statins reduce the level of membrane-bound active Ras/Rho proteins, thereby impairing corresponding functions. Since broad clinical experience already exists for statins, their incorporation into established tumor-therapeutic regimens would be realizable in a rather short period of time. Here, data available at present arguing for the usefulness of statins in anticancer therapy are summarized and discussed. 3. group C: bound to the extracelluar face of the plasma membrane by glycosylphosphatidylinositol;(dr. Antoine Sayegh clinic Aleppo ,Syria)
1.the amlodipine acts as Calcium channels blockers (CCB) which inhibits for the CA++ to enter intracellular from many mechanisms by the blocking the voltage channels dependent for the CA++ or from the acting on the receptor channels dependent for CA++ ,and from the other actions of the CCB on the relations between the CA++ and the IP3 which plays an important roles on its formation from the PIP2 to IP3 + DAG and on its receptors ( ip3)also from the effects of the CA++ on the ubiquitin proteasome pathway ,so from the last roles of the CCB which inhibits the actions of the CA++ on the relations between the CA++ and the IP3 and on the also IP3 receptors So we can we use the CCB for the treatment for the cancers ,because the IP3 and the CA++ with the relation with the calcineurin and the active NFAT help for the genes transcription , so ,we must study the patients which treated from the CCB and we must evaluate the ratios of the cancers in these groups of the patients ( the ratios in this group very very low ) ,also from the roles of the multiple kinase as the(calcium/calmodulin-dependent protein kinase (CaMK),with the other proteinkinase as protein kinase D (PKD), microtubule affinity-regulating kinase (MARK), salt-inducible kinase (SIK), checkpoint kinase-1 (CHK1) and other kinases) mediate specific phosphorylation of human histone deacetylase-4 (HDAC4) on three 14-3-3-binding sites. Myosin phosphatase-targeting subunit-1 (MYPT1)–protein phosphatase-1 (PP1) and PP2A can also act on these sites. The association of 14-3-3 proteins with HDAC4 retains it in the cytoplasm and prevents its interaction with transcription factors such as myocyte enhancer factor-2 (MEF2), thereby releasing these transcription factors for transcriptional activation. So when we inhibits the (calcium/calmodulin-dependent protein kinase (CaMK)which acts with other mechanisms in the signaling of the CA++ for the translations and for the transductions or transcription on the genes by the STAT or SMAD4 pathways
2. we use the angiotensin converting enzyme inhibitors (ACEI) with the CCB so to get the most important synergistic effects from their combination , and because the ACEI inhibits the actions of the Ang II ,and from the relation from the Ang II on the ATII receptor and on the ATI receptor especially also ( angiotensin I receptor) which excite the formation of the IP3 also from the PLC which acts to form the IP3 and the DAG from the PIP2,so the IP3+ the CA++ and the DAG activates the PKC which play as the vasoconstrictive roles in the hypertension ( HT) , so when we use the combination from the ACEI +CCB we get the best effects on the HT from the vasodilatation roles and from the indirect effects on the IP3 formation, so we can use the ACEI + CCB in the treatment of the cancer in the futures also .
3.the most important notices for the treatments for the cancer from two new ways : a. the neprilysin path ways :(from the mechanism of degradation ) which convert the GTP to c GMP with the CA++ which act as vasodilatation:
1.the first way: the neprilysin(nep) activate( degradation )the ANP which convert the GTP to c GMP .
2.the second way :the nep acts ( degradation) on the BK to BK2and the BK2 NOS convert the L Arg to NO (nitrous oxide ) which also convert the GTP to c GMP with the CA++ to dilate the vessels. So the drugs which activate the neprilysin pathways cause the vasodilatation with the important roles of the NO on the genes
2. the effects of the :
a. angiotensin converting enzyme inhibitors (ACEI)which decrease the breakdown of the bradykinin.
b. the calcium channels blockers (CCB ) which increase the synthesis of the bradykinin . the bradykinin which binds to the b2 kinin receptors which acts as:
1. on the coronary epithelium activate the intracellular NO synthase and to release the endothelium derived NO.
2.on the myocardium : diffuses of the NO in the myocytes and regulates the mitochondrial respiration .
But the NO in the tissues plays an important roles in the cancers because the macrophages :these cells activated by the cytokines or by the TNF which produces the nitric oxide ( NO ) which kills the organisms ( for the aids viruses also) and the malignant cells ( cancers )and the macrophages which aid and which did not aid also by the antibodies .
So the most important uses of the combination from the ACEI with the CCB in the future to treat the different cancers and for the different organisms like the aids also .
4. can we use the combination also for the treatment for HT as before between the ACEI plus angiotensin II receptor blockers( ARB) to get more synergistic effects when we face the genetic mutations on the ANG gene, Ang I and Ang I receptor genes ,or on the ADDI ( adducin gene )??? . Please answer me as fast as you can .
With the best regards from the dr. Antoine Sayegh The new dr. Antoine Sayegh declarations on the cancer treatments and for the aids also : My great dear: please contact me on these E-mails in the same time to confirm your sending of your e-mails to me
E-mails: antsay@aloola.sy dr.antoinesayegh@dcemail.com dr.antoine7sayegh@yahoo.com dr.antsay@gmail.com
the website :
http//:sayeghresearches.wetpaint.com
the page:
http://sayeghresearches.wetpaint.com/page/The+new++dr.+Antoine+Sayegh+declarations+on+the++cancer+treatments+%3A with the best regards from the dr. Antoine Sayegh
my great dear :the director of the cancer ,cardiology ,and Aids research centers
I hope your expensive evaluations for my new declarations on cancer treatments
,because always I find in you the honorable and the respectable
researchers of the world .
so I wait for your evaluations for my declarations as fast as you can
your faithfully dr. Antoine Sayegh
The new dr. Antoine Sayegh declarations on the cancer treatments :
I call all the cancer research centers of the world to concentrate on my new suggestions in their trials on cancer
1.the amlodipine acts as Calcium channels blockers (CCB) which inhibits for the CA++ to enter intracellular from many mechanisms by the blocking the voltage channels dependent for the CA++ or from the acting on the receptor channels dependent for CA++ ,and from the other actions of the CCB on the relations between the CA++ and the IP3 which plays an important roles on its formation from the PIP2 to IP3 + DAG and on its receptors ( ip3)also from the effects of the CA++ on the ubiquitin proteasome pathway ,so from the last roles of the CCB which inhibits the actions of the CA++ on the relations between the CA++ and the IP3 and on the also IP3 receptors So we can we use the CCB for the treatment for the cancers ,because the IP3 and the CA++ with the relation with the calcineurin and the active NFAT help for the genes transcription , so ,we must study the patients which treated from the CCB and we must evaluate the ratios of the cancers in these groups of the patients ( the ratios in this group very very low ) ,also from the roles of the multiple kinase as the(calcium/calmodulin-dependent protein kinase (CaMK),with the other proteinkinase as protein kinase D (PKD), microtubule affinity-regulating kinase (MARK), salt-inducible kinase (SIK), checkpoint kinase-1 (CHK1) and other kinases) mediate specific phosphorylation of human histone deacetylase-4 (HDAC4) on three 14-3-3-binding sites. Myosin phosphatase-targeting subunit-1 (MYPT1)–protein phosphatase-1 (PP1) and PP2A can also act on these sites. The association of 14-3-3 proteins with HDAC4 retains it in the cytoplasm and prevents its interaction with transcription factors such as myocyte enhancer factor-2 (MEF2), thereby releasing these transcription factors for transcriptional activation. So when we inhibits the (calcium/calmodulin-dependent protein kinase (CaMK)which acts with other mechanisms in the signaling of the CA++ for the translations and for the transductions or transcription on the genes by the STAT or SMAD4 pathways
2. we use the angiotensin converting enzyme inhibitors (ACEI) with the CCB so to get the most important synergistic effects from their combination , and because the ACEI inhibits the actions of the Ang II ,and from the relation from the Ang II on the ATII receptor and on the ATI receptor especially also ( angiotensin I receptor) which excite the formation of the IP3 also from the PLC which acts to form the IP3 and the DAG from the PIP2,so the IP3+ the CA++ and the DAG activates the PKC which play as the vasoconstrictive roles in the hypertension ( HT) , so when we use the combination from the ACEI +CCB we get the best effects on the HT from the vasodilatation roles and from the indirect effects on the IP3 formation, so we can use the ACEI + CCB in the treatment of the cancer in the futures also .
3.the most important notices for the treatments for the cancer from two new ways : a. the neprilysin path ways :(from the mechanism of degradation ) which convert the GTP to c GMP with the CA++ which act as vasodilatation:
1.the first way: the neprilysin(nep) activate( degradation )the ANP which convert the GTP to c GMP .
2.the second way :the nep acts ( degradation) on the BK to BK2and the BK2 NOS convert the L Arg to NO (nitrous oxide ) which also convert the GTP to c GMP with the CA++ to dilate the vessels. So the drugs which activate the neprilysin pathways cause the vasodilatation with the important roles of the NO on the genes
2. the effects of the :
a. angiotensin converting enzyme inhibitors (ACEI)which decrease the breakdown of the bradykinin.
b. the calcium channels blockers (CCB ) which increase the synthesis of the bradykinin . the bradykinin which binds to the b2 kinin receptors which acts as:
1. on the coronary epithelium activate the intracellular NO synthase and to release the endothelium derived NO.
2.on the myocardium : diffuses of the NO in the myocytes and regulates the mitochondrial respiration .
But the NO in the tissues plays an important roles in the cancers because the macrophages :these cells activated by the cytokines or by the TNF which produces the nitric oxide ( NO ) which kills the organisms ( for the aids viruses also) and the malignant cells ( cancers )and the macrophages which aid and which did not aid also by the antibodies .
So the most important uses of the combination from the ACEI with the CCB in the future to treat the different cancers and for the different organisms like the aids also .
4. can we use the combination also for the treatment for HT as before between the ACEI plus angiotensin II receptor blockers( ARB) to get more synergistic effects when we face the genetic mutations on the ANG gene, Ang I and Ang I receptor genes ,or on the ADDI ( adducin gene )??? . Please answer me as fast as you can .
With the best regards from the dr. Antoine Sayegh
dr. antoine sayegh researches Home dear the directors: for the aids ,cancer , genes medical centers
my great dears :
the directors for the aids , cancer and medical centers for genes deformities
your help and support for my theoretic researches : the new treatments for aids and cancer and genes deformities. Dear the professors which work in the medical research centers for aids ,cancer ,and genes deformities
you can choose the suitable parts of my researches for fund and trials making ,and you can change any part to be suitable for the trials ,and you can make the applications for the researches fund ,you are the coordinator for the applications and I am act as the cooperation in the researches due to the hard conditions for the fund applications ,and we can make a deal between us, and all the scientific rights are equal ratios between me and the centers ,and the fund directions because you can help millions of the patients with the fatal diseases ,please do not hesitate to contact me for the :
the web sites : http-sayeghresearches.wetpaint.com
http://sayeghresearches.wetpaint.com/page/dr.antoine+sayegh+researches+Home?mail=1130
the new e-mails : dr.antoinesayegh@yahoo.com antsay@aloola.sy
all the scientific rights are equal ratios between me and the centers ,and the fund directions
please do not hesitate to contact me as fast as you can
your faithfully dr .antoine sayegh . many thanks and greetings for the directions which send for me the e-mails to encourage me for more works and studies , for the professor dr. dr .f .c. sitzmann, for the directors and the staffs for the united nations ,and amnesty international ,WHO ,un foundation, hrw, all research centers, foundations ,associations ,agencies ,medical directions , and for the webinar of the AAA , for the care cancer , for GEN Genetic Engineering & Biotechnology webinar ,als webinar, for susan komen cure ,for national cancer institute ,nfcr,pei ,and for the staff of the homburg univercity ,proposal central review system and GLUCORE,and the wetpaint site and the yahoo mail and the google site and all friends. the important notes: 1.my dear: you can send the Email page for the research medical centers ,scientist ,researchers and patients with the fatal diseases. 2. if you find a good part of my researches , for the fund and the trials making , you can make a deal with me indirectly , and you can register it in any international directions like the united nation(UN) or the WHO to avoid any accusations and misunderstands in the future. 3. please visit my web site , the home page and the other pages , you will find many short stories ,its aims to excite the alive human feelings ,and to create again the ethical values in the human emotions which we need in our life ,to save our and the next generations genes , because there are no any benefits from the regrets in the future at all .
My great dears : the directors The director of the UNITED NATION MR.BAN KI MOON The director for the WHO DR.CHAN The director for the EUROPEAN COMMISSION The director for the MEDICAL RESEARCH CENTER The director for the INTERNATIONAL HUMAN RIGHTS The director for the INTERNATIONAL COURT OF JUSTICE The director for the INTERNATIONAL CRIMINAL COURT Many thanks and greetings for you and your staff My great dears : With the great love for my country Syria ,and with the great honor and the great respect for the president MR.DR.BASHAR AL ASSAD, I hope from you to forward for the president of Syria all the researches and all the e-mails and all the scientific stories also which I had forwarded from me to you ,to avoid any misunderstand in the future, because my researches did not contain any military or politically or terrorist issues ,but it contains many theoretic researches for the treatments for the fatal diseases ,like the aids ,the cancer ,and the genes deformities ,to help millions of the patients in the world ,and to give them the new hopes in their life .
We ask you to protect the life of the scientists and the researchers against any harms may face them in their fate ,and for their kinsman also under the severe ,hard ,and strong resolutions from the united nation ,because they must realize, that the blood of the scientists not very cheap ,but very expensive ,and ask also the governments for the scientist in every country to save the life for them and protect them against any harms ( artificial car accidents ,threaten for children kidnapped ,create false evidences , certificates and illegal relations , penetrate the own places of work ,house, computers, whispering in your ears you are agent and traitor for a foreign countries and deal with foreign centers, and the artificial troubles and the problems from the annoying, boring, disturbing, wearisome ,inconvenient ,and troublesome neighbors ,which challenge against the scientists works ). and they are responsible for any types of the harms ( the high levels: the president ,the parties ,the intelligences and the governments), and must open an international investigations after the exposure to any harms .
my researches did not contain any military ,political, or terrorist issues
but it contains very important projects for helping the patients with fatal diseases ,the aids and cancer and genes deformities, and the relations with the foreign centers( for aids and cancer and gene therapy ) around the world must give the scientists the honor and they must also proud of their deals as medals ,and the order of merits on their chest , and they must not shame from it , and I hope for my researches to find the help and support for them with the warm hearts ,and to avoid any miss understand in the future ,because all Syrian websites closed in front of my researches .and these web sites are :
health-min@net.sy
info@shern.net
baath@baath-party.org
mhe@shem.net
info@parliament.gov.sy I had send for the Syrian web sites many e-mails with respectable words which express my polite behavior , you can see one of the e-mail in the photo gallery under the name( dr. sayegh document) in the web site documentshttp://sayeghresearches.wetpaint.com/page/dr.antoine+sayegh+researches+Home?mail=1130 if the man do not give his benefits for his parents and own nation, he shall not give any benefits for the others . My great dears we ask the god to put between your hands the peace and the health because you are the free and noble men in the world and you have alive conscious to save the live of the scientists from any dangers or harms around the world ,and our human civilization ,so who can protect and save them except you??? My great dears : between your faithful hands the most expensive values in the world 1.our fantastic planet ( the Earth)which full of different types of THE LIFE 2. the admirable structure of the DNA which save our inheritances . 3.the real treasures, the scientists . 4. my researches as an engineering sketch of the fantastic palace which needs its creation in the future My great dears ,with the increase of the fatal infections, like the hepatitis ( especially the type C) and the aids diseases in the world ,so our human civilization is threaten with the other fatal disease in the future ,as I mentioned in many paragraphs and in my researches part I and part II ,especially in the saymann syndrome ,as a strong proofs from the facts we live for my researches and my comments also , because of the many viruses qualities with the sever and strong virulence ,and due to its properties of the hyper variable antigens , we shall face in our fates in the future more of the danger viruses, if we did not stop the abnormal sexual actions with many partners , so the best decisions and resolutions from the united nations ( UN ) and the WHO, only able to save our life and our human civilization ,my dears please trust and believe in my researches ,which acts as important alerts for our life. With the increase of the fatal diseases in the world like the aids and hepatitis c ,and we shall face in the future more of the oncogenes viruses due to the abnormal sexual actions with many partners ( as in saymann syndrome ),and I hope to wipe the tears from the eyes of the parents ,while their children suffer from the aids or cancers ,so we can ignite a small candle in our hands to light our deep dark roads by the exchange the medical informations for all the scientists around the world ,and fund the good researches for making the trials ,and if you prefer to distribute my researches for all medical center in the world for all the countries without any exceptions, because it is better for me to spend the money for fund the researches, than to spends it for new guns industrializations which destroy our human civilization, because the peace brings the benefits and the war brings the blood ,and the blood brings the blood ,the tortures and the pains . With the blesses of the Jesus I hope for you good health ,nice wishes, fine days with happiness my great dears: I hope your help and support to make the international day against the debauchery and the prostitution actions and the terrorisms , and the revenue ( around the world) from the beneficent assemblies and from the donations from the voluntary festivals in this day or from the revenue of new medical report against the intoxications go for : 1.for fund the researches against aids and cancer and genes deformities under director of the UN. 50% of the donations. 2. for the international organizations for the international human rights for the health.50% of the donations. The real facts from the abnormal sexual actions which causes the aids and cancer and genes deformities may harm and wound the feelings and the emotions of these nets( the abnormal sexual groups the ***** women and the adulterer men which named aldababir and the great hornets ((which addict the prostitution actions until their bones )) which act as the wailing wolf with high cries from false wounds while the victim between its jaws ,wrestle against the death ,and the UN and WHO must take off the teeth from these savage jaws) ,and they act as one big family, and all the members are equals between them ,from the different types , the poor and the rich, the different religions types and the different social levels ,,under one union, the great prostitution family , they throw their nets around their victims ,and they invert the real facts the white black and the black white so they are traitors: 1. for them self and for their children and for their nations because they are the nearer in blood by destroy their genes and the life for them and for the next generations genes because they are the nearer bloody nets 2. because they make the hard troubles against the scientists by deprive them from the human sciences progresses in their researches and the traveling out of their countries for the participation in the international conferences by artificial disruptions under the pretext of the national duties , and the scientists pay their life for the science progress to find new treatments for the fatal diseases ,so their work came from their love for their nations to save millions life of the patients( especially to protect the life of the children for these nets) and to help the human civilization from the extermination ,so the respect for the scientists and the save for every rights for them and nominate them for international prizes are the best gifts for them because they are the treasure for the human civilization and they hold in their minds top medical scientific knowledge as the icy mountains the great parts of it hidden under the water When any city be under the typhoon strikes, after a while may recovers from it, but the hits from the viral attacks from the abnormal sexual actions with many partners ,may not recover at all and for ever. The abnormal sexual actions with many partners help for the increase the cancers in the future and help to change the endemic disease to epidemic diseases from their travel from one country to another , so these nets must not protected from any governmental or non governmental organizations , parties ,intelligences ,special religion groups ( the big first disaster, if these nets are parts of these groups and the second dangerous disaster when the legal partner from marriage, may deal with these nets or his kinsman or the relatives to him ,agents for these nets which put the scientists under their mercy and control the life of the scientists from their orders ) and did not hide their actions directly or indirectly because these nets are the best and fertile ground for the masked and secret crimes and terrorist actions because these nets work in its eyries ,and their hand full of copious blood and crimes ,and the blood bring the blood ,and may be from the sarcasm and mockery of the fate ,the nets of the prostitution actions save and protect the general laws ,the human ethics, and the moral behavior ,as the popular examples :who protected it ,was who stole it ,and this is the greatest disaster in our life . The prophylaxis from the fatal diseases which came from the immediate stop of the abnormal sexual actions with many partners and the save for the women the real freedom and liberation them from these actions as slaves ,and not to sell their bodies in the slave trade market, and to respect them and love them as mothers ,wives , sisters, daughters ,and these facts not come from racialism or racism but from absolute medical and scientific rules. so what is this life with stand and stare ,we must challenge the evil and dare ,and save the life under the laws of the UN and WHO and care a. The effects of the environmental changes on the genes are the most important for the next generations by 1. direct effects : the damage and the mutations 2. indirect effects : by the mediators 3. by feed back mechanisms between the genes 4. by the broken chromosomes from the radiations . b. the sever environmental effects on the genes causes sever harms for the next generations and for many decades due to fixed mutations or dysfunctional genes . c. the harm effects like the atomic irradiations and the climate disasters which causes the endemic or epidemic diseases or direct harmful from the rays of the sun like the sever lights dermatitis or squamous cell and basal cell carcinomas in the skin of the climate changes . d. the abnormal sexual actions which help for negative effects on the genes also help with these others factors for sever damage for the next generation genes and to eradicate the human genes from its normal roots. e. the sever environmental changes like the climate changes help for negative effects on the human genes by direct effects and cause the fixed mutations or by stop the actions of many genes for short or long times and these effects inherited to the next generations and without any changes in the nucleic acids series ( normal genes ), so the dysfunctions of these genes not related to its structures but to its change the relation between the DNA and its histones ,it means the environmental effects did not harm the DNA structure but change the relations between the genes and its histones and help for dysfunction of the genes , so the effects of the circumstances around the genes help for its actions and help for the balance between the genes also and this important notice explain the harm effects on the nations for long times and the structure of the nucleic acids did not changed , so the response from the genes for the environmental changes different from one genes to another , so the bad circumstances around the genes may stay for many decades without DNA changes ,but the changes between the genes and between the genes and the histones explain for the new relations ( like the uses or addictions)between them changed for long times . and this new important notice is a strong proof for my theory. The real fixed facts is the abnormal sexual action is the first killer in the world by destroy our genes and the next generation genes and causes of the cancers and genes deformities and by destroy any genes help for the other killer diseases indirectly and these facts are obvious as the sun rays even if we ignore it or deny it And if these nets(abnormal sexual nets)sowing the colocynth, they will get the bitter. . The importance of these researches to stop immediately the abnormal sexual actions because it destroy our genes and the next generations genes and the multi mutations help for loose of formations proteins or receptors or any important factor may help in immune system weakness and give great chance for new infections or renewed ancient infections which help for many old or new epidemic fatal and dangerous diseases in the future because of the loose many factors can protect the bodies from these diseases , so the create the new departments for the international human rights of the health is very important to save our life and for the next generations ,we must confess freely and frankly if we did not stop the abnormal sexual actions immediately ,so our life and our next generations in sever dangerous in the future and must put these sexual nets under international laws because( often ) these nets, its hands full of blood and crimes. the increase of the aids and cancer and genes deformities in the future due to abnormal sexual actions did not promise us for nice future for the next generations and in these nets in every family we find one of the chronic infections , aids cancer and genes diseases in high ratios and every disease with reverse analysis we find high new mutations are not found in their parents before ,it means these abrupt mutations are dangerous for our genes and for the next generations and we need many decades after the stop of the abnormal sexual actions to clean our genes and the genes for the next generations. The increase of the ratios of the aids and cancers and genes diseases in the future may give us bad effects on the number of the peoples in the world and the curve of the number of the peoples increase slowly and achieve the apex and from these diseases the curve decrease sharply and quickly in the future .. My great dears :please answer me for my requests and help and support my the researches .and to make a great international campaigns against the debauchery and the prostitution actions in the day ( 17 /1/ every year in the feast of the great SAINT ANTOINE ) Or the day ( 13/6/ every year in the feast of the of the SAINT ANTOINE de PADOUE) as the international days against aids or the smoking .because these campaigns challenge against the prostitution and debauchery actions and against also the crimes and the terrorisms ,and come what come may, they must pay ( the abnormal sexual nets)in the promise day under the sever international laws from the UN and the WHO which can save our life and the next generations and the organizations ,the governmental or non governmental which use these nets for secret aims ,they must be responsible under the international laws ,and the international protections for the scientists save their life and their works against any harms ,and any traps may deform their personalities to make around them the doubts and suspicious actions to destroy them and to loose many of the chances for vacancies ,and the opportunities for funds for their projects, and the international silence give a good chance for these nets for the secrets crimes and assassinations ,and terrorist actions. My gear dears your answers for my requests save the life for the scientists and make the lights in their efforts ,and your sever , strong, very fast, and right decisions can stop immediately these nets ,so I hope in the sooner time hearing your high voice( the voices of the freedom and the justice ) around the world against these nets. I hope Your positive answer to help me and support me in my researches that you send for me more e-mails to encourage me for more work and studies and to challenge together hands by hands , the aids and the cancer and the genes deformities and to ask the directors of the UN and the WHO for more strong decisions and hard and sever international laws to save our life and the next generations and the life of the scientists and their families also , and save for them the human international rights against harms and for their researches, and protect them under international laws , and put new department for : (the international human rights for the health ) and its duties 1. for protections and prophylaxis : a. make general scan for the peoples in many places in the world for DNA assays and for search for the antibodies partners and for the detection for the genital organisms . b. search for the abnormal sexual nets and put them under international laws c. save the life for the children and the second legal partner ( from marriage) and their human rights .and advice the parents with fatal diseases like aids or genes deformities from birth a new children with many genes deformities and fatal diseases and change the life of the nets to normal life and stop immediately the prostitutions actions and find for them new jobs ,under the care of the governments organizations , and non governments organizations, for the labour offices, or the beneficent assemblies ,in their countries d. support the international researches for aids and cancer and genes deformities to facilitate to find new treatments and help for any trial scan save the patients life . e . put new laws to save our life and our generations from fatal diseases and .from chronic intoxications and from crash syndrome .due to the physical harms on the human bodies . f. we can use also the osteocytes genes for determines the chronic intoxication by chemical materials in the food or the coffee or in the internal clothes, like the white cement( we must search for the sio2 , and the so3, and the MG toxcity in the blood) ,or the detergents ,or any organic chemicals, and not discovered by the direct detections or when it gives negative false results after the run of the time or after the treatments with many drugs ( anti hyper tension or the chemo therapy ) as the irritants which makes cancers or blood pressure elevation chemical materials which form the harms for the diet genes OOG1 also , so we can discover its toxic harms by formation for all drugs and chemicals the genetic maps to find its harm on the genes in the alive cells to get the difference from the organisms and the rays harms by detect the types mutations in the series in the nucleic acids changes for the genes TP53,BRCA2,XRCC1,ATM,MCIR. and also we make for the same specimen in the cadaver or alive patients the genetic maps for the old cells and the new formed cells in the same tissue , and we must study the high formations of the renal cysts( false cysts which different from the inherited genetic types ) due to the irritants by its accumulation in the renal glomeruli and cause the damage in it ,so we must search for the new renal cysts with haematuria to search for the chemical irritants . So the DNA assays for the genes GSTM1,GSTT1,NQO1,NAT2,CYP1A1,TS,OGG!,and other genes which help for detection for the chemical chronic intoxications g. give the help and the support for all medical centers in the word and advice them with the presses to challenge the prostitution actions . h. one of the most important duties ,the save for the general International human rights , the UN and the WHO ,and the organization for the HUMAN RIGHTS must make randomly legitimate investigations for the dead peoples around the world and in the graves also for the causes of the death and for the DNA changes ( polymorphisms )in a different times . 2. for the scientists : a. help for the researchers and the scientists for registration in the WHO health departments b. make registration for proposal researches and save for them their scientific rights . c. help for the medical researches fund to facilitate the trials d .ask the governments for the scientist in every country to save the life for them and protect them against any harms e. save for the scientist their life under international laws from the united nation against any artificial harms (any types which controlled or directed from these nets , so the scientists may be good targets for them by changing their life as in the hell) for them and for their families .and open international investigations after exposure to any harms . f. help the scientists to improve their circumstances and help them in finding good jobs to get more benefits from them and to excite them for more work and studies 3.this important department the human rights for the health of the WHO can help for the decrease the infective fatal diseases and challenge the cancer and the genes deformities and directed also from the united nation to facilitate its action in the world .. and put these nets ( of any nature )or groups(must not be protected locally) of the abnormal sexual actions under these laws and they must hold great responsibilities in the future , and the history with great love save for the directors of the UN, the WHO, the EUROPEAN COMMISSION , the , MEDICAL RESEARCH CENTER ,and the INTERNATIONAL HUMAN RIGHTS their names with glory many thanks and many greetings for the directors for the UN ,the WHO, the European commission ,research centers, and for the international human rights and for their staff. Your faithfully: dr. antoine sayegh
with the best regards from the dr. Antoine Sayegh
http://antsay.webs.com/apps/photos http://sayeghresearches.wetpaint.com/photo/11223556/internatinal+certificate http://www.iainternalmeds.com/certificate.6128612.html
The newest dr. Antoine Sayegh declarations on the cancer treatments and aids also :
I call all the cancer research centers of the world to concentrate on my new suggestions in their trials on cancer and on aids also : We must use the combinations from many drugs which act on the ( Myristate , farensyl ,glucosylphosphatidylinositol ) in the same time in the protection from cancers which promise us for the new the treatments for the aids and the cancer also. The membrane-attached proteins divided into three groups: 1.group A : bound to the cytosolic face of the plasma membrane by myristate as v-Src is a kind of non-receptor protein tyrosine kinase involved in cell signaling: 1.we must get more benefits from the properties of the Dihydropyridine-type calcium-channel antagonists (DTCCA) which plays an important roles in many diseases , because they decrease vasospastic propensity. These drugs are suspected to have anti-oxidant properties in other diseases as in the systemic sclerosis . that nifedipine and nicardipine decrease circulating markers of oxidative stress damage in patients.. the effects of nifedipine on O2•- release from human monocytes, on protein phosphorylation with phorbol myristate acetate (PMA) as stimulator and on protein kinase C (PKC) activity, This beneficial property of nifedipine seems to be mediated by its cellular action and by the inhibition of PKC activity,so we can use this drugs against the diseases which associate with the oxidative harms. ( Paris V University, Cochin Hospital, Cochin Institute,france) 2.we must get more benefits also from the relations from the Phorbol 12-Myristate 13-acetate (PMA) and angiotensin II (Ang II)which increased both the percentage of scavenger receptors Scr-positive cells and the Scr mean fluorescence intensity. PMA and Ang II also increased Scr mRNA and promoter activity in a time- and dose-responsive manner. Protein kinase C and calmodulin transduction pathways were involved in Scr up-regulation induced by PMA and Ang II. Additionally, a serine/threonine kinase was involved in PMA stimulation. Functional analysis showed that both AP-1 and ets motifs were specific response elements to PMA stimulation in HMCLs ,so the drugs which change the relations between the PMA and the ANGII which inhibits the actions of the ANGII can breaks down the transduction pathways (Center for Nephrology, Royal Free and University College Medical School, Royal Free Campus, London, England, United Kingdom) 2. group B:bound to the cytosolic face of the plasma membrane by farnesyl as Ras protein which also plays a key role in cell signaling: From my participation with Webinar on the antitumor effects of the biophosphonate in breast cancer 1. The gene on the chromosome 1 and the location q22 encodes an enzyme that catalyzes the production of geranyl pyrophosphate and farnesyl pyrophosphate from isopentenyl pyrophosphate and dimethylallyl pyrophosphate. The resulting product, farnesyl pyrophosphate, a substrate for protein farnesylation and geranylgeranylation, Drugs Zoledronic acid is an amino-bisphosphonate that inhibit this enzyme FPP [farnesyl diphosphate] synthase prevent the post-translational modifications of small GTPases and have been used to treat diseases related to bone resorption. Multiple pseudogenes have been found on chromosomes 1, 7, 14, 15, 21 and X. Multiple transcript variants encoding different isoforms have been found for this gene .so we can can the Bisphosphonates as clodronate and zoledronic acid to inhibit also the other isoforms . 2.the second point to avoid the the harm effects on the normal cells from the uses of the Bisphosphonates as clodronate and zoledronic acid , we can use the antibodies against the FPP [farnesyl diphosphate] synthase as in my theoretic researches for temporary periods instead of the biophosphonates Please visit the all researches I and all researches II on my website http://sayeghresearches.wetpaint.com/ http://sayeghresearches.wetpaint.com/forum 3.from the effects of the uses of the biophosphonates on the relation the FPP [farnesyl diphosphate] synthase as a molecular partner for p13(II) and G4 accessory proteins which opens new prospects for treatment of retrovirus-induced leukemia HTLV1 p13(II) not on cancer but on the retroviruses also 4, the HMG-CoA reductase inhibitors (statins) as an anticancer drugs: from the international researches we found : ( University of Mainz, Germany ) Apart from their lipid lowering activity, HMG-CoA reductase inhibitors (statins) impair numerous cellular functions associated with metastasis, e.g. gene expression, angiogenesis, cell adhesion, cell motility and invasiveness. Furthermore, statins have impact on apoptotic cell death and modulate cellular susceptibility to cell killing by anticancer drugs and ionizing radiation. Part of the effects provoked by statins are due to the inhibition of the prenylation of low molecular weight GTPases, in particular Ras and Rho, which play key roles in signaling evoked by stimulation of cell surface receptors. C-terminal lipid modification of Ras/Rho GTPases is essential for their correct intracellular localization and function. By depletion of the cellular pool of isoprene precursor molecules, statins reduce the level of membrane-bound active Ras/Rho proteins, thereby impairing corresponding functions. Since broad clinical experience already exists for statins, their incorporation into established tumor-therapeutic regimens would be realizable in a rather short period of time. Here, data available at present arguing for the usefulness of statins in anticancer therapy are summarized and discussed. 3. group C: bound to the extracelluar face of the plasma membrane by glycosylphosphatidylinositol;(dr. Antoine Sayegh clinic Aleppo ,Syria) 1.the amlodipine acts as Calcium channels blockers (CCB) which inhibits for the CA++ to enter intracellular from many mechanisms by the blocking the voltage channels dependent for the CA++ or from the acting on the receptor channels dependent for CA++ ,and from the other actions of the CCB on the relations between the CA++ and the IP3 which plays an important roles on its formation from the PIP2 to IP3 + DAG and on its receptors ( ip3)also from the effects of the CA++ on the ubiquitin proteasome pathway ,so from the last roles of the CCB which inhibits the actions of the CA++ on the relations between the CA++ and the IP3 and on the also IP3 receptors So we can we use the CCB for the treatment for the cancers ,because the IP3 and the CA++ with the relation with the calcineurin and the active NFAT help for the genes transcription , so ,we must study the patients which treated from the CCB and we must evaluate the ratios of the cancers in these groups of the patients ( the ratios in this group very very low ) ,also from the roles of the multiple kinase as the(calcium/calmodulin-dependent protein kinase (CaMK),with the other proteinkinase as protein kinase D (PKD), microtubule affinity-regulating kinase (MARK), salt-inducible kinase (SIK), checkpoint kinase-1 (CHK1) and other kinases) mediate specific phosphorylation of human histone deacetylase-4 (HDAC4) on three 14-3-3-binding sites. Myosin phosphatase-targeting subunit-1 (MYPT1)–protein phosphatase-1 (PP1) and PP2A can also act on these sites. The association of 14-3-3 proteins with HDAC4 retains it in the cytoplasm and prevents its interaction with transcription factors such as myocyte enhancer factor-2 (MEF2), thereby releasing these transcription factors for transcriptional activation. So when we inhibits the (calcium/calmodulin-dependent protein kinase (CaMK)which acts with other mechanisms in the signaling of the CA++ for the translations and for the transductions or transcription on the genes by the STAT or SMAD4 pathways
2. we use the angiotensin converting enzyme inhibitors (ACEI) with the CCB so to get the most important synergistic effects from their combination , and because the ACEI inhibits the actions of the Ang II ,and from the relation from the Ang II on the ATII receptor and on the ATI receptor especially also ( angiotensin I receptor) which excite the formation of the IP3 also from the PLC which acts to form the IP3 and the DAG from the PIP2,so the IP3+ the CA++ and the DAG activates the PKC which play as the vasoconstrictive roles in the hypertension ( HT) , so when we use the combination from the ACEI +CCB we get the best effects on the HT from the vasodilatation roles and from the indirect effects on the IP3 formation, so we can use the ACEI + CCB in the treatment of the cancer in the futures also .
3.the most important notices for the treatments for the cancer from two new ways : a. the neprilysin path ways :(from the mechanism of degradation ) which convert the GTP to c GMP with the CA++ which act as vasodilatation:
1.the first way: the neprilysin(nep) activate( degradation )the ANP which convert the GTP to c GMP .
2.the second way :the nep acts ( degradation) on the BK to BK2and the BK2 NOS convert the L Arg to NO (nitrous oxide ) which also convert the GTP to c GMP with the CA++ to dilate the vessels. So the drugs which activate the neprilysin pathways cause the vasodilatation with the important roles of the NO on the genes
2. the effects of the :
a. angiotensin converting enzyme inhibitors (ACEI)which decrease the breakdown of the bradykinin.
b. the calcium channels blockers (CCB ) which increase the synthesis of the bradykinin . the bradykinin which binds to the b2 kinin receptors which acts as:
1. on the coronary epithelium activate the intracellular NO synthase and to release the endothelium derived NO.
2.on the myocardium : diffuses of the NO in the myocytes and regulates the mitochondrial respiration .
But the NO in the tissues plays an important roles in the cancers because the macrophages :these cells activated by the cytokines or by the TNF which produces the nitric oxide ( NO ) which kills the organisms ( for the aids viruses also) and the malignant cells ( cancers )and the macrophages which aid and which did not aid also by the antibodies .
So the most important uses of the combination from the ACEI with the CCB in the future to treat the different cancers and for the different organisms like the aids also .
4. can we use the combination also for the treatment for HT as before between the ACEI plus angiotensin II receptor blockers( ARB) to get more synergistic effects when we face the genetic mutations on the ANG gene, Ang I and Ang I receptor genes ,or on the ADDI ( adducin gene )??? . Please answer me as fast as you can The newest relations between the Calcium channels blockers (CCB) and the angiotensin converting enzyme inhibitors (ACEI) with the transcription factor nuclear factor E2-related factor 2 (Nrf2) directly or indirectly as anti oxidant or anti cancer The proofs : 1. Nrf2 is a protein messenger contained in every cell of the body that sends information to the cell’s DNA, When this protein messenger called Nrf2 is activated, it enters the nucleus of every cell and it turns on several hundred of the DNA genes. And the genes that it turns on, or turns up, are known collectively as “survival genes.” These genes enable cells to survive in the face of several different kinds of stress, especially oxidative stress which is due to the over production of free radicals and other oxidants. many researches confirm my declaration of the link : WikiGenes - NFE2L2 - nuclear factor (erythroid-derived 2)-like 2.mht 2. Flunarizine induces Nrf2-mediated transcriptional activation of heme oxygenase-1 in protection of auditory cells from cisplatin H-S So, H-J Kim, J-H Lee, J-H Lee, S-Y Park, C Park, Y-H Kim, J-K Kim, K-M Lee, K-S Kim, S-Y Chung, W-C Jang, S-K Moon, H-T Chung and R-K ParPretreatment with flunarizine predominantly induced the transcriptional expression of HO-1 among other Nrf2-regulated genes in cisplatin-treated cells. (a) Cells were treated with 20 M cisplatin for the indicated periods in the presence or absence of 10 M flunarizine. Then, total RNA was isolated by RNAzol and cDNA was synthesized by reverse transcriptase. The Nrf2-related genes, including HO-1, NQO1, GCLC, GCLM, GST-1, and GSTA4, were amplified with specific primer sets, respectively. Also, cells were treated with 20 M cisplatin, 10 M flunaizine and various doses of SnPPIX for 36 h and used to measure the cell viability (b), to determine the enzymatic activity of HO-1 at 20 h (c), or to test the expression level of HO-1 protein by Western blotting (d). # P<0.01, ** P<0.01 by one-way ANOVA, compared with only cisplatin treated group (#) or flunarizine/cisplatin-cotreated group (*, **). (e) To examine the effect of calcium channel blockers on the HO-1 induction, cells were treated with various calcium channel blockers for 24 h. Cell lysates were separated on 12% SDS-PAGE to probe for HO-1 and -actin by Western blot. Nicardipine, 10 M; nifedipine, 12.5 M; ditiazem, 10 M; pimozide, 2.5 M 3. Nitric oxide activation of Keap1/Nrf2 signaling in human colon carcinoma cells Chun-Qi Li Min Young Kim Luiz C. Godoy Apinya Thiantanawat Laura J. Trudel Gerald N. Wogan The transcription factor NF-E2-related nuclear factor 2 (Nrf2) regulates expression of genes that protect cells from oxidative damage. Here, we characterized nitric oxide (•NO)-induced Nrf2–Kelch-like ECH-associated protein 1 (Keap1) signaling and its role in counteracting •NO-induced apoptosis of human colon cancer HCT116 cells. Nrf2 was localized in the cytoplasm in control cells; •NO triggered its rapid nuclear accumulation, transcriptional activation, and up-regulation of HO-1, NQO1, and GCL, but not GST A4 and P1 subunits. Nrf2 accumulation in the nucleus was also associated with enhanced transcription and posttranscriptional modifications. (S)-nitrosation of Keap1 may contribute to nuclear accumulation of Nrf2 by facilitating its dissociation from Keap1, thus initiating •NO-mediated Nrf2–Keap1 signaling. •NO-mediated induction of ARE-dependent genes occurred well before apoptosis, as judged by caspase 3 activation. Collectively, these results show that the Nrf2–Keap1 signaling pathway mediates protective cellular responses to mitigate •NO-induced damage and may contribute to the relative resistance of HCT116 to •NO-induced cytotoxicity. 4.the researches from iHOP - Information Hyperlinked over Proteins [ NPPA ].mht a. To verify the hypothesis that the angiotensin converting enzyme (ACE) level may affect the metabolism of circulating atrial natriuretic factor (ANF), the acute and chronic effects of benazepril on plasma ANF levels were studied in hypertensive patients under basal conditions and in response to acute volume expansion b. Atrial natriuretic peptide (ANP ), C-type natriuretic peptide, and C-ANP-(4-23), a ligand for the natriuretic peptide clearance receptor, equipotently inhibited production of VEGF by as much as 88% and inhibited ET- or hypoxia-stimulated VEGF transcription c. Atrial natriuretic peptide(ANP) has been shown to reduce tumor necrosis factor-alpha (TNF-alpha)-induced activation of endothelial cells via inhibition of p38 mitogen-activated protein kinase (MAPK ) and nuclear factor (NF)-kappaB pathways. d. Atrial natriuretic factor 1-28 (ANF) and C-type natriuretic factor-22 (CNP) reduced angiotensin II (Ang II)- and platelet-derived growth factor-stimulated PAI-1 mRNA expression in rat aortic smooth muscle cells by 50% to 70%, with corresponding reductions in PAI-1 protein release e. Arial natriuretic factor ANP evoked production of superoxide was found to activate c-Jun N-terminal kinase (JNK). f. Atrial natriuretic peptide (ANP)-C receptor activation has been shown to inhibit adenylyl cyclase (AC) activity as well as to stimulate phospholipase C (PLC) signaling pathways. 5.researches from : WikiGenes - Evolutionary Knowledge.mht 1. NFE2L2 - nuclear factor (erythroid-derived 2)-like 2 Gene: induction via Nrf2 activation in hepatoma cells. Early effects( only 1 h of exposure) of Disease relevance of NFE2L2 Human prx1 gene is a target of Nrf2 and is up- regulated by hypoxia/ reoxygenation: implication to tumor biology. Neuroblastoma cells were stably transfected with DN- Nrf2, which/ Synonyms: NRF2 2. MAPKAP1 - mitogen-activated protein kinase... Gene: Physical interactions of MAPKAP1 Transfection of PC12 cells with dominant- negative Nrf2 abolished the SIN- 1- induced increase in Nrf2- ARE binding and subsequent upregulation of HO- 1 expression, leading neuroblastoma cells( TAM- 67 cells) to apoptosis induced by the nitric oxide( NO) 3. Hmox1 - heme oxygenase (decycling) 1 Gene: stress induced the nuclear import of Nrf2 and the binding of Nrf2 to the HO- 1 antioxidant and inducible nitric oxide synthase( iNOS) expression in the aortas of ovariectomized rats, and the regulatory heme oxygenase( HO) and calcium- dependent nitric oxide synthase( cNOS) activities were increased
With the best regards from the dr. Antoine Sayegh The new dr. Antoine Sayegh methods for treatments from the stem cells: http://sayeghresearches.wetpaint.com/page/The+new+dr.+Antoine+Sayegh+methods+for+treatments+from+the+stem+cells%3A http://antsay.webs.com/ the aims for the treatments : 1.to use the new methods for the treatments of the chronic and for the fatal diseases also. 2.for the new hopes in the treatments for the different cancers from the killer cells( K cells ,LAK cells ), in the bone marrow suppressions ,and for the aids also from the new created of the CD4 cells . 3.to replace the harmed cells in the different tissues from the infective diseases . 4.to open the new treatments in the future for the hereditary diseases. The sources of the stem cells 1. from placenta. 2. from the adults bone marrow and from the adipose tissues . 3. from the cloning from the somatic cell nucleus transfer to the unucleated ovum as a new totipotent cells which form the blastocyte which gave us the new stem cells . 4.the methods of the dr. James Thompson : to get the pluripotent cells from the blostocyte. 5. the methods of the dr. John Gearhart :to get the stem cells from the embryonic germ cells . The methods which isolate and purify human of the different stem cells from the bone marrow or from the adipose tissues or from other sources, which use different materials for the isolation and for the culture for these cells in the different steps 1. for the processing of the bone marrow mononuclear (adipose , placental) cells. 2. for the magnetic activated cell sorting and for the fluorescence activated cells sorting also. 3.for the culture of the human monoclonal bone marrow cells. These methods use many harmful materials for the stem cells which weak its metabolic vitalities and can change its normal properties or qualities to shortening its ages and help for the deviation toward the oncogene positions ( as carcinogenic cells in the recipient tissues ) . The new dr. Antoine Sayegh methods as below: The first step :the preparing of the isolated and purified stem cells from the bone marrow: a. we take the bone marrow from the iliac crest from the human being . b. we divide the specimen into two parts : 1. we inject the first part in the blood of the animal to form against every component the antibodies the IGM and the IGG to use them later . 2. we incubate the second part (of the bone marrow ) with the animal antibodies which we got it from the first step ( the serum of the animal ) ,and in the same times we incubate the second part with the anti anti bodies the hyper variable part prepared against the anti bodies for the target cells which we need for the isolation like the CD34 or the CD38( from the known isolated cells ) to destroy all the unneeded cells and to protect the target cells . *****The preparation of the hyper variable part of the anti –antibodies from the ( known isolated target cells like CD34 ,CD38, or for ES embryonic stem cells): a. we get the antibodies against the markers proteins ( against the known makers proteins of the target cell from any person as the SSEA-3 (Stage-specific embryonic antigen) ,SEA-4,TRA-1-60(Tumor rejection antigen-1) ,TRA-1-81 or the markers of the CD34 ,CD38 ) b. we incubate part of these antibodies with the pepsin to get the Fab(ab)2 the binding antigen sites ,and the other part with the papain to get the fab(ab)1 and the fragment c (FC)to get different types of the FAB parts with the different types of the FC also in the same times .. c. we incubate the bone marrow of the patient with the different types of the antibodies residues (held on latex) with the different suitable complements proteins to form heavy immune complexes ,we centrifuge the complexes to get from the layer of the latex the target cells . d. we isolate the target cells from the complexes from the cooling or heating , or from the changes of the ph ,so we get the weakened target cells to use them later to form from them only the anti antibodies. e. we can use instead of the latex ,the red blood cells held on its surfaces the hyper variable part of the antibodies against the different types of the FABs which act as the target antigens to form with the bone marrow of the patients with different FABs residue with complements proteins also the agglutinations forms of the immune response to collect between its nets the weakened target cells . 3. we incubate and culture the residues of the second part to get more of the target cells with its high vital activities while the other cells weakened and destroyed from the different antibodies from the uses of the STAT 3 factor and Oct-4 transcription factor which activate the LIF receptor to activate LIF –STAT 3 Signaling pathway which promotes Embryonic Stem cell self-renewal . . 4. we isolate the residues from the low speeds by the centrifuge to avoid the harms for the target cells to get the pure target cells because when these cells were cultured it gained very huge masses which precipitate it easily to the bottom of the tube. 5. we wash the cultured cells from the saline or from not harmful fluids (buffers) many times to clean it from the other ineffective attached cells. The second step: 1. we inject the patients from the vaccine( under the skin) which composed from the anti antibodies( the hyper variable parts of the antibodies ) against the target cells ( CD34,CD38 ,other cells like Embryonic Stem Cells) before many days of the uses of the stem cells ,to destroy every anti bodies against the target cells from the immune response of the uses of the stem cells.. 2. we inject the patients from the new purified stem cells with the low numbers ( not millions but hundreds thousands ) to decrease the cost( money payments) of the treatments . 3.we help the recipient tissues to receive the new stem cells with the low numbers ( hundreds thousands ) and to elongate its ages and to embrace into the defected tissues as a new normal cells from: the next third step : to confirm the new actions of the injected stem cells : a. by the injection of the angiogenesis factors into the blood of the patients , as FGFs , FGF1( the potent factors for angiogenesis ) and as FGF2 , VEGF factors also and from the TGF beta to help the stem cells to invade the recipient tissues . b. we inject into the blood of the patients also the helper the HGF and the MET which activates the proliferations of the stem cells with the E-cadherine, or from the uses of the ET-1 ( endotheline-1) which help the stem cells to be accepted from the target tissues . so the new treatments from the new methods of the isolated stem cells promise us for the best treatments for the fatal diseases in the future which act near the normal cells mechanisms to re repair the defected ( failed ) tissues or organs . My new E-mails: antsay@aloola.sy dr.antoinesayegh@gmail.com dr.antoine7sayegh@dcemail.com dr.antoine7sayegh@yahoo.com dr.antsay@gmail.com dr.antoinesayegh@mail.com the websites : http//:sayeghresearches.wetpaint.com http//:antsay.webs.com http://www.facebook.com/people/Antoine-Sayegh/100002063614016 with the best regards from the dr. Antoine Sayegh
The newest dr. Antoine Sayegh declarations on the cancer treatments and for the aids also : http://sayeghresearches.wetpaint.com/page/The+newest++dr.+Antoine+Sayegh+declarations+on+the+cancer+treatments+and+for+the+aids+also+%3A My great dear: please contact me on these E-mails in the same time to confirm your sending of your e-mails to me
E-mails: antsay@aloola.sy dr.antoinesayegh@dcemail.com dr.antoine7sayegh@yahoo.com dr.antsay@gmail.com
the websites :
http//:sayeghresearches.wetpaint.com http//:antsay.webs.com
with the best regards from the dr. Antoine Sayegh
The newest dr. Antoine Sayegh declarations on the cancer treatments and aids also :
I call all the cancer research centers of the world to concentrate on my new suggestions in their trials on cancer and on aids also : We must use the combinations from many drugs which act on the ( Myristate , farensyl ,glucosylphosphatidylinositol ) in the same time in the protection from cancers and and which promise us for the new the treatments for the aids and the cancer also. The membrane-attached proteins divided into three groups: 1.group A : bound to the cytosolic face of the plasma membrane by myristate as v-Src is a kind of non-receptor protein tyrosine kinase involved in cell signaling: 1.we must get more benefits from the properties of the Dihydropyridine-type calcium-channel antagonists (DTCCA) which plays an important roles in many diseases , because they decrease vasospastic propensity. These drugs are suspected to have anti-oxidant properties in other diseases as in the systemic sclerosis . that nifedipine and nicardipine decrease circulating markers of oxidative stress damage in patients.. the effects of nifedipine on O2•- release from human monocytes, on protein phosphorylation with phorbol myristate acetate (PMA) as stimulator and on protein kinase C (PKC) activity, This beneficial property of nifedipine seems to be mediated by its cellular action and by the inhibition of PKC activity,so we can use this drugs against the diseases which associate with the oxidative harms. ( Paris V University, Cochin Hospital, Cochin Institute,france) 2.we must get more benefits also from the relations from the Phorbol 12-Myristate 13-acetate (PMA) and angiotensin II (Ang II)which increased both the percentage of scavenger receptors Scr-positive cells and the Scr mean fluorescence intensity. PMA and Ang II also increased Scr mRNA and promoter activity in a time- and dose-responsive manner. Protein kinase C and calmodulin transduction pathways were involved in Scr up-regulation induced by PMA and Ang II. Additionally, a serine/threonine kinase was involved in PMA stimulation. Functional analysis showed that both AP-1 and ets motifs were specific response elements to PMA stimulation in HMCLs ,so the drugs which change the relations between the PMA and the ANGII which inhibits the actions of the ANGII can breaks down the transduction pathways (Center for Nephrology, Royal Free and University College Medical School, Royal Free Campus, London, England, United Kingdom) 2. group B:bound to the cytosolic face of the plasma membrane by farnesyl as Ras protein which also plays a key role in cell signaling: From my participation with Webinar on the antitumor effects of the biophosphonate in breast cancer 1. The gene on the chromosome 1 and the location q22 encodes an enzyme that catalyzes the production of geranyl pyrophosphate and farnesyl pyrophosphate from isopentenyl pyrophosphate and dimethylallyl pyrophosphate. The resulting product, farnesyl pyrophosphate, a substrate for protein farnesylation and geranylgeranylation, Drugs Zoledronic acid is an amino-bisphosphonate that inhibit this enzyme FPP [farnesyl diphosphate] synthase prevent the post-translational modifications of small GTPases and have been used to treat diseases related to bone resorption. Multiple pseudogenes have been found on chromosomes 1, 7, 14, 15, 21 and X. Multiple transcript variants encoding different isoforms have been found for this gene .so we can can the Bisphosphonates as clodronate and zoledronic acid to inhibit also the other isoforms . 2.the second point to avoid the the harm effects on the normal cells from the uses of the Bisphosphonates as clodronate and zoledronic acid , we can use the antibodies against the FPP [farnesyl diphosphate] synthase as in my theoretic researches for temporary periods instead of the biophosphonates Please visit the all researches I and all researches II on my website http://sayeghresearches.wetpaint.com/ http://sayeghresearches.wetpaint.com/forum 3.from the effects of the uses of the biophosphonates on the relation the FPP [farnesyl diphosphate] synthase as a molecular partner for p13(II) and G4 accessory proteins which opens new prospects for treatment of retrovirus-induced leukemia HTLV1 p13(II) not on cancer but on the retroviruses also 4, the HMG-CoA reductase inhibitors (statins) as an anticancer drugs: from the international researches we found : ( University of Mainz, Germany ) Apart from their lipid lowering activity, HMG-CoA reductase inhibitors (statins) impair numerous cellular functions associated with metastasis, e.g. gene expression, angiogenesis, cell adhesion, cell motility and invasiveness. Furthermore, statins have impact on apoptotic cell death and modulate cellular susceptibility to cell killing by anticancer drugs and ionizing radiation. Part of the effects provoked by statins are due to the inhibition of the prenylation of low molecular weight GTPases, in particular Ras and Rho, which play key roles in signaling evoked by stimulation of cell surface receptors. C-terminal lipid modification of Ras/Rho GTPases is essential for their correct intracellular localization and function. By depletion of the cellular pool of isoprene precursor molecules, statins reduce the level of membrane-bound active Ras/Rho proteins, thereby impairing corresponding functions. Since broad clinical experience already exists for statins, their incorporation into established tumor-therapeutic regimens would be realizable in a rather short period of time. Here, data available at present arguing for the usefulness of statins in anticancer therapy are summarized and discussed. 3. group C: bound to the extracelluar face of the plasma membrane by glycosylphosphatidylinositol;(dr. Antoine Sayegh clinic Aleppo ,Syria)
1.the amlodipine acts as Calcium channels blockers (CCB) which inhibits for the CA++ to enter intracellular from many mechanisms by the blocking the voltage channels dependent for the CA++ or from the acting on the receptor channels dependent for CA++ ,and from the other actions of the CCB on the relations between the CA++ and the IP3 which plays an important roles on its formation from the PIP2 to IP3 + DAG and on its receptors ( ip3)also from the effects of the CA++ on the ubiquitin proteasome pathway ,so from the last roles of the CCB which inhibits the actions of the CA++ on the relations between the CA++ and the IP3 and on the also IP3 receptors So we can we use the CCB for the treatment for the cancers ,because the IP3 and the CA++ with the relation with the calcineurin and the active NFAT help for the genes transcription , so ,we must study the patients which treated from the CCB and we must evaluate the ratios of the cancers in these groups of the patients ( the ratios in this group very very low ) ,also from the roles of the multiple kinase as the(calcium/calmodulin-dependent protein kinase (CaMK),with the other proteinkinase as protein kinase D (PKD), microtubule affinity-regulating kinase (MARK), salt-inducible kinase (SIK), checkpoint kinase-1 (CHK1) and other kinases) mediate specific phosphorylation of human histone deacetylase-4 (HDAC4) on three 14-3-3-binding sites. Myosin phosphatase-targeting subunit-1 (MYPT1)–protein phosphatase-1 (PP1) and PP2A can also act on these sites. The association of 14-3-3 proteins with HDAC4 retains it in the cytoplasm and prevents its interaction with transcription factors such as myocyte enhancer factor-2 (MEF2), thereby releasing these transcription factors for transcriptional activation. So when we inhibits the (calcium/calmodulin-dependent protein kinase (CaMK)which acts with other mechanisms in the signaling of the CA++ for the translations and for the transductions or transcription on the genes by the STAT or SMAD4 pathways
2. we use the angiotensin converting enzyme inhibitors (ACEI) with the CCB so to get the most important synergistic effects from their combination , and because the ACEI inhibits the actions of the Ang II ,and from the relation from the Ang II on the ATII receptor and on the ATI receptor especially also ( angiotensin I receptor) which excite the formation of the IP3 also from the PLC which acts to form the IP3 and the DAG from the PIP2,so the IP3+ the CA++ and the DAG activates the PKC which play as the vasoconstrictive roles in the hypertension ( HT) , so when we use the combination from the ACEI +CCB we get the best effects on the HT from the vasodilatation roles and from the indirect effects on the IP3 formation, so we can use the ACEI + CCB in the treatment of the cancer in the futures also .
3.the most important notices for the treatments for the cancer from two new ways : a. the neprilysin path ways :(from the mechanism of degradation ) which convert the GTP to c GMP with the CA++ which act as vasodilatation:
1.the first way: the neprilysin(nep) activate( degradation )the ANP which convert the GTP to c GMP .
2.the second way :the nep acts ( degradation) on the BK to BK2and the BK2 NOS convert the L Arg to NO (nitrous oxide ) which also convert the GTP to c GMP with the CA++ to dilate the vessels. So the drugs which activate the neprilysin pathways cause the vasodilatation with the important roles of the NO on the genes
2. the effects of the :
a. angiotensin converting enzyme inhibitors (ACEI)which decrease the breakdown of the bradykinin.
b. the calcium channels blockers (CCB ) which increase the synthesis of the bradykinin . the bradykinin which binds to the b2 kinin receptors which acts as:
1. on the coronary epithelium activate the intracellular NO synthase and to release the endothelium derived NO.
2.on the myocardium : diffuses of the NO in the myocytes and regulates the mitochondrial respiration .
But the NO in the tissues plays an important roles in the cancers because the macrophages :these cells activated by the cytokines or by the TNF which produces the nitric oxide ( NO ) which kills the organisms ( for the aids viruses also) and the malignant cells ( cancers )and the macrophages which aid and which did not aid also by the antibodies .
So the most important uses of the combination from the ACEI with the CCB in the future to treat the different cancers and for the different organisms like the aids also .
4. can we use the combination also for the treatment for HT as before between the ACEI plus angiotensin II receptor blockers( ARB) to get more synergistic effects when we face the genetic mutations on the ANG gene, Ang I and Ang I receptor genes ,or on the ADDI ( adducin gene )??? . Please answer me as fast as you can .
With the best regards from the dr. Antoine Sayegh The new dr. Antoine Sayegh declarations on the cancer treatments and for the aids also : My great dear: please contact me on these E-mails in the same time to confirm your sending of your e-mails to me
E-mails: antsay@aloola.sy dr.antoinesayegh@dcemail.com dr.antoine7sayegh@yahoo.com dr.antsay@gmail.com
the website :
http//:sayeghresearches.wetpaint.com
the page:
http://sayeghresearches.wetpaint.com/page/The+new++dr.+Antoine+Sayegh+declarations+on+the++cancer+treatments+%3A with the best regards from the dr. Antoine Sayegh
my great dear :the director of the cancer ,cardiology ,and Aids research centers
I hope your expensive evaluations for my new declarations on cancer treatments
,because always I find in you the honorable and the respectable
researchers of the world .
so I wait for your evaluations for my declarations as fast as you can
your faithfully dr. Antoine Sayegh
The new dr. Antoine Sayegh declarations on the cancer treatments :
I call all the cancer research centers of the world to concentrate on my new suggestions in their trials on cancer
1.the amlodipine acts as Calcium channels blockers (CCB) which inhibits for the CA++ to enter intracellular from many mechanisms by the blocking the voltage channels dependent for the CA++ or from the acting on the receptor channels dependent for CA++ ,and from the other actions of the CCB on the relations between the CA++ and the IP3 which plays an important roles on its formation from the PIP2 to IP3 + DAG and on its receptors ( ip3)also from the effects of the CA++ on the ubiquitin proteasome pathway ,so from the last roles of the CCB which inhibits the actions of the CA++ on the relations between the CA++ and the IP3 and on the also IP3 receptors So we can we use the CCB for the treatment for the cancers ,because the IP3 and the CA++ with the relation with the calcineurin and the active NFAT help for the genes transcription , so ,we must study the patients which treated from the CCB and we must evaluate the ratios of the cancers in these groups of the patients ( the ratios in this group very very low ) ,also from the roles of the multiple kinase as the(calcium/calmodulin-dependent protein kinase (CaMK),with the other proteinkinase as protein kinase D (PKD), microtubule affinity-regulating kinase (MARK), salt-inducible kinase (SIK), checkpoint kinase-1 (CHK1) and other kinases) mediate specific phosphorylation of human histone deacetylase-4 (HDAC4) on three 14-3-3-binding sites. Myosin phosphatase-targeting subunit-1 (MYPT1)–protein phosphatase-1 (PP1) and PP2A can also act on these sites. The association of 14-3-3 proteins with HDAC4 retains it in the cytoplasm and prevents its interaction with transcription factors such as myocyte enhancer factor-2 (MEF2), thereby releasing these transcription factors for transcriptional activation. So when we inhibits the (calcium/calmodulin-dependent protein kinase (CaMK)which acts with other mechanisms in the signaling of the CA++ for the translations and for the transductions or transcription on the genes by the STAT or SMAD4 pathways
2. we use the angiotensin converting enzyme inhibitors (ACEI) with the CCB so to get the most important synergistic effects from their combination , and because the ACEI inhibits the actions of the Ang II ,and from the relation from the Ang II on the ATII receptor and on the ATI receptor especially also ( angiotensin I receptor) which excite the formation of the IP3 also from the PLC which acts to form the IP3 and the DAG from the PIP2,so the IP3+ the CA++ and the DAG activates the PKC which play as the vasoconstrictive roles in the hypertension ( HT) , so when we use the combination from the ACEI +CCB we get the best effects on the HT from the vasodilatation roles and from the indirect effects on the IP3 formation, so we can use the ACEI + CCB in the treatment of the cancer in the futures also .
3.the most important notices for the treatments for the cancer from two new ways : a. the neprilysin path ways :(from the mechanism of degradation ) which convert the GTP to c GMP with the CA++ which act as vasodilatation:
1.the first way: the neprilysin(nep) activate( degradation )the ANP which convert the GTP to c GMP .
2.the second way :the nep acts ( degradation) on the BK to BK2and the BK2 NOS convert the L Arg to NO (nitrous oxide ) which also convert the GTP to c GMP with the CA++ to dilate the vessels. So the drugs which activate the neprilysin pathways cause the vasodilatation with the important roles of the NO on the genes
2. the effects of the :
a. angiotensin converting enzyme inhibitors (ACEI)which decrease the breakdown of the bradykinin.
b. the calcium channels blockers (CCB ) which increase the synthesis of the bradykinin . the bradykinin which binds to the b2 kinin receptors which acts as:
1. on the coronary epithelium activate the intracellular NO synthase and to release the endothelium derived NO.
2.on the myocardium : diffuses of the NO in the myocytes and regulates the mitochondrial respiration .
But the NO in the tissues plays an important roles in the cancers because the macrophages :these cells activated by the cytokines or by the TNF which produces the nitric oxide ( NO ) which kills the organisms ( for the aids viruses also) and the malignant cells ( cancers )and the macrophages which aid and which did not aid also by the antibodies .
So the most important uses of the combination from the ACEI with the CCB in the future to treat the different cancers and for the different organisms like the aids also .
4. can we use the combination also for the treatment for HT as before between the ACEI plus angiotensin II receptor blockers( ARB) to get more synergistic effects when we face the genetic mutations on the ANG gene, Ang I and Ang I receptor genes ,or on the ADDI ( adducin gene )??? . Please answer me as fast as you can .
With the best regards from the dr. Antoine Sayegh
dr. antoine sayegh researches Home dear the directors: for the aids ,cancer , genes medical centers
my great dears :
the directors for the aids , cancer and medical centers for genes deformities
your help and support for my theoretic researches : the new treatments for aids and cancer and genes deformities. Dear the professors which work in the medical research centers for aids ,cancer ,and genes deformities
you can choose the suitable parts of my researches for fund and trials making ,and you can change any part to be suitable for the trials ,and you can make the applications for the researches fund ,you are the coordinator for the applications and I am act as the cooperation in the researches due to the hard conditions for the fund applications ,and we can make a deal between us, and all the scientific rights are equal ratios between me and the centers ,and the fund directions because you can help millions of the patients with the fatal diseases ,please do not hesitate to contact me for the :
the web sites : http-sayeghresearches.wetpaint.com
http://sayeghresearches.wetpaint.com/page/dr.antoine+sayegh+researches+Home?mail=1130
the new e-mails : dr.antoinesayegh@yahoo.com antsay@aloola.sy
all the scientific rights are equal ratios between me and the centers ,and the fund directions
please do not hesitate to contact me as fast as you can
your faithfully dr .antoine sayegh . many thanks and greetings for the directions which send for me the e-mails to encourage me for more works and studies , for the professor dr. dr .f .c. sitzmann, for the directors and the staffs for the united nations ,and amnesty international ,WHO ,un foundation, hrw, all research centers, foundations ,associations ,agencies ,medical directions , and for the webinar of the AAA , for the care cancer , for GEN Genetic Engineering & Biotechnology webinar ,als webinar, for susan komen cure ,for national cancer institute ,nfcr,pei ,and for the staff of the homburg univercity ,proposal central review system and GLUCORE,and the wetpaint site and the yahoo mail and the google site and all friends. the important notes: 1.my dear: you can send the Email page for the research medical centers ,scientist ,researchers and patients with the fatal diseases. 2. if you find a good part of my researches , for the fund and the trials making , you can make a deal with me indirectly , and you can register it in any international directions like the united nation(UN) or the WHO to avoid any accusations and misunderstands in the future. 3. please visit my web site , the home page and the other pages , you will find many short stories ,its aims to excite the alive human feelings ,and to create again the ethical values in the human emotions which we need in our life ,to save our and the next generations genes , because there are no any benefits from the regrets in the future at all .
My great dears : the directors The director of the UNITED NATION MR.BAN KI MOON The director for the WHO DR.CHAN The director for the EUROPEAN COMMISSION The director for the MEDICAL RESEARCH CENTER The director for the INTERNATIONAL HUMAN RIGHTS The director for the INTERNATIONAL COURT OF JUSTICE The director for the INTERNATIONAL CRIMINAL COURT Many thanks and greetings for you and your staff My great dears : With the great love for my country Syria ,and with the great honor and the great respect for the president MR.DR.BASHAR AL ASSAD, I hope from you to forward for the president of Syria all the researches and all the e-mails and all the scientific stories also which I had forwarded from me to you ,to avoid any misunderstand in the future, because my researches did not contain any military or politically or terrorist issues ,but it contains many theoretic researches for the treatments for the fatal diseases ,like the aids ,the cancer ,and the genes deformities ,to help millions of the patients in the world ,and to give them the new hopes in their life .
We ask you to protect the life of the scientists and the researchers against any harms may face them in their fate ,and for their kinsman also under the severe ,hard ,and strong resolutions from the united nation ,because they must realize, that the blood of the scientists not very cheap ,but very expensive ,and ask also the governments for the scientist in every country to save the life for them and protect them against any harms ( artificial car accidents ,threaten for children kidnapped ,create false evidences , certificates and illegal relations , penetrate the own places of work ,house, computers, whispering in your ears you are agent and traitor for a foreign countries and deal with foreign centers, and the artificial troubles and the problems from the annoying, boring, disturbing, wearisome ,inconvenient ,and troublesome neighbors ,which challenge against the scientists works ). and they are responsible for any types of the harms ( the high levels: the president ,the parties ,the intelligences and the governments), and must open an international investigations after the exposure to any harms .
my researches did not contain any military ,political, or terrorist issues
but it contains very important projects for helping the patients with fatal diseases ,the aids and cancer and genes deformities, and the relations with the foreign centers( for aids and cancer and gene therapy ) around the world must give the scientists the honor and they must also proud of their deals as medals ,and the order of merits on their chest , and they must not shame from it , and I hope for my researches to find the help and support for them with the warm hearts ,and to avoid any miss understand in the future ,because all Syrian websites closed in front of my researches .and these web sites are :
health-min@net.sy
info@shern.net
baath@baath-party.org
mhe@shem.net
info@parliament.gov.sy I had send for the Syrian web sites many e-mails with respectable words which express my polite behavior , you can see one of the e-mail in the photo gallery under the name( dr. sayegh document) in the web site documentshttp://sayeghresearches.wetpaint.com/page/dr.antoine+sayegh+researches+Home?mail=1130 if the man do not give his benefits for his parents and own nation, he shall not give any benefits for the others . My great dears we ask the god to put between your hands the peace and the health because you are the free and noble men in the world and you have alive conscious to save the live of the scientists from any dangers or harms around the world ,and our human civilization ,so who can protect and save them except you??? My great dears : between your faithful hands the most expensive values in the world 1.our fantastic planet ( the Earth)which full of different types of THE LIFE 2. the admirable structure of the DNA which save our inheritances . 3.the real treasures, the scientists . 4. my researches as an engineering sketch of the fantastic palace which needs its creation in the future My great dears ,with the increase of the fatal infections, like the hepatitis ( especially the type C) and the aids diseases in the world ,so our human civilization is threaten with the other fatal disease in the future ,as I mentioned in many paragraphs and in my researches part I and part II ,especially in the saymann syndrome ,as a strong proofs from the facts we live for my researches and my comments also , because of the many viruses qualities with the sever and strong virulence ,and due to its properties of the hyper variable antigens , we shall face in our fates in the future more of the danger viruses, if we did not stop the abnormal sexual actions with many partners , so the best decisions and resolutions from the united nations ( UN ) and the WHO, only able to save our life and our human civilization ,my dears please trust and believe in my researches ,which acts as important alerts for our life. With the increase of the fatal diseases in the world like the aids and hepatitis c ,and we shall face in the future more of the oncogenes viruses due to the abnormal sexual actions with many partners ( as in saymann syndrome ),and I hope to wipe the tears from the eyes of the parents ,while their children suffer from the aids or cancers ,so we can ignite a small candle in our hands to light our deep dark roads by the exchange the medical informations for all the scientists around the world ,and fund the good researches for making the trials ,and if you prefer to distribute my researches for all medical center in the world for all the countries without any exceptions, because it is better for me to spend the money for fund the researches, than to spends it for new guns industrializations which destroy our human civilization, because the peace brings the benefits and the war brings the blood ,and the blood brings the blood ,the tortures and the pains . With the blesses of the Jesus I hope for you good health ,nice wishes, fine days with happiness my great dears: I hope your help and support to make the international day against the debauchery and the prostitution actions and the terrorisms , and the revenue ( around the world) from the beneficent assemblies and from the donations from the voluntary festivals in this day or from the revenue of new medical report against the intoxications go for : 1.for fund the researches against aids and cancer and genes deformities under director of the UN. 50% of the donations. 2. for the international organizations for the international human rights for the health.50% of the donations. The real facts from the abnormal sexual actions which causes the aids and cancer and genes deformities may harm and wound the feelings and the emotions of these nets( the abnormal sexual groups the ***** women and the adulterer men which named aldababir and the great hornets ((which addict the prostitution actions until their bones )) which act as the wailing wolf with high cries from false wounds while the victim between its jaws ,wrestle against the death ,and the UN and WHO must take off the teeth from these savage jaws) ,and they act as one big family, and all the members are equals between them ,from the different types , the poor and the rich, the different religions types and the different social levels ,,under one union, the great prostitution family , they throw their nets around their victims ,and they invert the real facts the white black and the black white so they are traitors: 1. for them self and for their children and for their nations because they are the nearer in blood by destroy their genes and the life for them and for the next generations genes because they are the nearer bloody nets 2. because they make the hard troubles against the scientists by deprive them from the human sciences progresses in their researches and the traveling out of their countries for the participation in the international conferences by artificial disruptions under the pretext of the national duties , and the scientists pay their life for the science progress to find new treatments for the fatal diseases ,so their work came from their love for their nations to save millions life of the patients( especially to protect the life of the children for these nets) and to help the human civilization from the extermination ,so the respect for the scientists and the save for every rights for them and nominate them for international prizes are the best gifts for them because they are the treasure for the human civilization and they hold in their minds top medical scientific knowledge as the icy mountains the great parts of it hidden under the water When any city be under the typhoon strikes, after a while may recovers from it, but the hits from the viral attacks from the abnormal sexual actions with many partners ,may not recover at all and for ever. The abnormal sexual actions with many partners help for the increase the cancers in the future and help to change the endemic disease to epidemic diseases from their travel from one country to another , so these nets must not protected from any governmental or non governmental organizations , parties ,intelligences ,special religion groups ( the big first disaster, if these nets are parts of these groups and the second dangerous disaster when the legal partner from marriage, may deal with these nets or his kinsman or the relatives to him ,agents for these nets which put the scientists under their mercy and control the life of the scientists from their orders ) and did not hide their actions directly or indirectly because these nets are the best and fertile ground for the masked and secret crimes and terrorist actions because these nets work in its eyries ,and their hand full of copious blood and crimes ,and the blood bring the blood ,and may be from the sarcasm and mockery of the fate ,the nets of the prostitution actions save and protect the general laws ,the human ethics, and the moral behavior ,as the popular examples :who protected it ,was who stole it ,and this is the greatest disaster in our life . The prophylaxis from the fatal diseases which came from the immediate stop of the abnormal sexual actions with many partners and the save for the women the real freedom and liberation them from these actions as slaves ,and not to sell their bodies in the slave trade market, and to respect them and love them as mothers ,wives , sisters, daughters ,and these facts not come from racialism or racism but from absolute medical and scientific rules. so what is this life with stand and stare ,we must challenge the evil and dare ,and save the life under the laws of the UN and WHO and care a. The effects of the environmental changes on the genes are the most important for the next generations by 1. direct effects : the damage and the mutations 2. indirect effects : by the mediators 3. by feed back mechanisms between the genes 4. by the broken chromosomes from the radiations . b. the sever environmental effects on the genes causes sever harms for the next generations and for many decades due to fixed mutations or dysfunctional genes . c. the harm effects like the atomic irradiations and the climate disasters which causes the endemic or epidemic diseases or direct harmful from the rays of the sun like the sever lights dermatitis or squamous cell and basal cell carcinomas in the skin of the climate changes . d. the abnormal sexual actions which help for negative effects on the genes also help with these others factors for sever damage for the next generation genes and to eradicate the human genes from its normal roots. e. the sever environmental changes like the climate changes help for negative effects on the human genes by direct effects and cause the fixed mutations or by stop the actions of many genes for short or long times and these effects inherited to the next generations and without any changes in the nucleic acids series ( normal genes ), so the dysfunctions of these genes not related to its structures but to its change the relation between the DNA and its histones ,it means the environmental effects did not harm the DNA structure but change the relations between the genes and its histones and help for dysfunction of the genes , so the effects of the circumstances around the genes help for its actions and help for the balance between the genes also and this important notice explain the harm effects on the nations for long times and the structure of the nucleic acids did not changed , so the response from the genes for the environmental changes different from one genes to another , so the bad circumstances around the genes may stay for many decades without DNA changes ,but the changes between the genes and between the genes and the histones explain for the new relations ( like the uses or addictions)between them changed for long times . and this new important notice is a strong proof for my theory. The real fixed facts is the abnormal sexual action is the first killer in the world by destroy our genes and the next generation genes and causes of the cancers and genes deformities and by destroy any genes help for the other killer diseases indirectly and these facts are obvious as the sun rays even if we ignore it or deny it And if these nets(abnormal sexual nets)sowing the colocynth, they will get the bitter. . The importance of these researches to stop immediately the abnormal sexual actions because it destroy our genes and the next generations genes and the multi mutations help for loose of formations proteins or receptors or any important factor may help in immune system weakness and give great chance for new infections or renewed ancient infections which help for many old or new epidemic fatal and dangerous diseases in the future because of the loose many factors can protect the bodies from these diseases , so the create the new departments for the international human rights of the health is very important to save our life and for the next generations ,we must confess freely and frankly if we did not stop the abnormal sexual actions immediately ,so our life and our next generations in sever dangerous in the future and must put these sexual nets under international laws because( often ) these nets, its hands full of blood and crimes. the increase of the aids and cancer and genes deformities in the future due to abnormal sexual actions did not promise us for nice future for the next generations and in these nets in every family we find one of the chronic infections , aids cancer and genes diseases in high ratios and every disease with reverse analysis we find high new mutations are not found in their parents before ,it means these abrupt mutations are dangerous for our genes and for the next generations and we need many decades after the stop of the abnormal sexual actions to clean our genes and the genes for the next generations. The increase of the ratios of the aids and cancers and genes diseases in the future may give us bad effects on the number of the peoples in the world and the curve of the number of the peoples increase slowly and achieve the apex and from these diseases the curve decrease sharply and quickly in the future .. My great dears :please answer me for my requests and help and support my the researches .and to make a great international campaigns against the debauchery and the prostitution actions in the day ( 17 /1/ every year in the feast of the great SAINT ANTOINE ) Or the day ( 13/6/ every year in the feast of the of the SAINT ANTOINE de PADOUE) as the international days against aids or the smoking .because these campaigns challenge against the prostitution and debauchery actions and against also the crimes and the terrorisms ,and come what come may, they must pay ( the abnormal sexual nets)in the promise day under the sever international laws from the UN and the WHO which can save our life and the next generations and the organizations ,the governmental or non governmental which use these nets for secret aims ,they must be responsible under the international laws ,and the international protections for the scientists save their life and their works against any harms ,and any traps may deform their personalities to make around them the doubts and suspicious actions to destroy them and to loose many of the chances for vacancies ,and the opportunities for funds for their projects, and the international silence give a good chance for these nets for the secrets crimes and assassinations ,and terrorist actions. My gear dears your answers for my requests save the life for the scientists and make the lights in their efforts ,and your sever , strong, very fast, and right decisions can stop immediately these nets ,so I hope in the sooner time hearing your high voice( the voices of the freedom and the justice ) around the world against these nets. I hope Your positive answer to help me and support me in my researches that you send for me more e-mails to encourage me for more work and studies and to challenge together hands by hands , the aids and the cancer and the genes deformities and to ask the directors of the UN and the WHO for more strong decisions and hard and sever international laws to save our life and the next generations and the life of the scientists and their families also , and save for them the human international rights against harms and for their researches, and protect them under international laws , and put new department for : (the international human rights for the health ) and its duties 1. for protections and prophylaxis : a. make general scan for the peoples in many places in the world for DNA assays and for search for the antibodies partners and for the detection for the genital organisms . b. search for the abnormal sexual nets and put them under international laws c. save the life for the children and the second legal partner ( from marriage) and their human rights .and advice the parents with fatal diseases like aids or genes deformities from birth a new children with many genes deformities and fatal diseases and change the life of the nets to normal life and stop immediately the prostitutions actions and find for them new jobs ,under the care of the governments organizations , and non governments organizations, for the labour offices, or the beneficent assemblies ,in their countries d. support the international researches for aids and cancer and genes deformities to facilitate to find new treatments and help for any trial scan save the patients life . e . put new laws to save our life and our generations from fatal diseases and .from chronic intoxications and from crash syndrome .due to the physical harms on the human bodies . f. we can use also the osteocytes genes for determines the chronic intoxication by chemical materials in the food or the coffee or in the internal clothes, like the white cement( we must search for the sio2 , and the so3, and the MG toxcity in the blood) ,or the detergents ,or any organic chemicals, and not discovered by the direct detections or when it gives negative false results after the run of the time or after the treatments with many drugs ( anti hyper tension or the chemo therapy ) as the irritants which makes cancers or blood pressure elevation chemical materials which form the harms for the diet genes OOG1 also , so we can discover its toxic harms by formation for all drugs and chemicals the genetic maps to find its harm on the genes in the alive cells to get the difference from the organisms and the rays harms by detect the types mutations in the series in the nucleic acids changes for the genes TP53,BRCA2,XRCC1,ATM,MCIR. and also we make for the same specimen in the cadaver or alive patients the genetic maps for the old cells and the new formed cells in the same tissue , and we must study the high formations of the renal cysts( false cysts which different from the inherited genetic types ) due to the irritants by its accumulation in the renal glomeruli and cause the damage in it ,so we must search for the new renal cysts with haematuria to search for the chemical irritants . So the DNA assays for the genes GSTM1,GSTT1,NQO1,NAT2,CYP1A1,TS,OGG!,and other genes which help for detection for the chemical chronic intoxications g. give the help and the support for all medical centers in the word and advice them with the presses to challenge the prostitution actions . h. one of the most important duties ,the save for the general International human rights , the UN and the WHO ,and the organization for the HUMAN RIGHTS must make randomly legitimate investigations for the dead peoples around the world and in the graves also for the causes of the death and for the DNA changes ( polymorphisms )in a different times . 2. for the scientists : a. help for the researchers and the scientists for registration in the WHO health departments b. make registration for proposal researches and save for them their scientific rights . c. help for the medical researches fund to facilitate the trials d .ask the governments for the scientist in every country to save the life for them and protect them against any harms e. save for the scientist their life under international laws from the united nation against any artificial harms (any types which controlled or directed from these nets , so the scientists may be good targets for them by changing their life as in the hell) for them and for their families .and open international investigations after exposure to any harms . f. help the scientists to improve their circumstances and help them in finding good jobs to get more benefits from them and to excite them for more work and studies 3.this important department the human rights for the health of the WHO can help for the decrease the infective fatal diseases and challenge the cancer and the genes deformities and directed also from the united nation to facilitate its action in the world .. and put these nets ( of any nature )or groups(must not be protected locally) of the abnormal sexual actions under these laws and they must hold great responsibilities in the future , and the history with great love save for the directors of the UN, the WHO, the EUROPEAN COMMISSION , the , MEDICAL RESEARCH CENTER ,and the INTERNATIONAL HUMAN RIGHTS their names with glory many thanks and many greetings for the directors for the UN ,the WHO, the European commission ,research centers, and for the international human rights and for their staff. Your faithfully: dr. antoine sayegh
antsay |
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| Started By | Thread Subject | Replies | Last Post | ||
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| Anonymous | Global weather | 0 | Oct 23 2010, 7:44 AM EDT by Anonymous | ||
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Thread started: Oct 23 2010, 7:44 AM EDT
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Rain snow and a global map means http://www.theweatherland.com/
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| Anonymous | The human civilization without the conscious and the ethics part2 | 1 | Sep 28 2009, 8:28 AM EDT by Anonymous | ||
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Thread started: Jan 8 2009, 6:47 AM EST
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and the innocent children in the station of the buses, and the dead peoples were the hundreds ,because there are no peace between the two races and the wars bring the bloods and the bloods bring the bloods ,the tortures ,the pains, the death for the all nations in the world, the pilot wounded in the battle , he needs the blood transfusion, and the great surprise was his blood group and his DNA analysis were different from his parents ,and he was from a different race ,and the analysis of the residues of the second young man also from the explosion was very strange ,so every young man kills his nation from the different ways ,this is the sarcasm and the mockery of the fates ,at last, we ask the noble men, in the world ,from the united nations and from the international organizations for the human rights to act with their alive conscious to stop the wars in the world and to prevent it also, to act like the white doves which hold the olive branches to bring the peace for the world ,and to put all the wars makers which their hands stained with the copious bloods of the innocent peoples and the innocent children under the international laws from the international criminal courts.
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| Anonymous | The human civilization without the conscious and the ethics part1 | 0 | Jan 8 2009, 6:46 AM EST by Anonymous | ||
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Thread started: Jan 8 2009, 6:46 AM EST
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The human civilization without the conscious and the ethics:
From sixty years ago ,in the great city, two neighbors families from different races, their two husbands were friends, their wives were pregnant in the same time ,they entered the obstetrics hospital for their birth, they bring their first child with physiologic jaundice ,the nurses put their newborns in the incubator for the treatments with the special rays to destroy the jaundice, by the mistake they change the names of the newborns due to great resemblance between them, so, the names exchanges changes their fates and their lives also, after many years the wars happened between the two races in the city, so they divide their nice city between them into two parts ,the first young man was the famous military pilot in the army ,and the second young man was the famous director in the secret resistance group, there were between them continuous bloody conflicts, the war between them were very terrible, the pilot bombs his enemy with the severe and the fatal bombs, the blood flowing from the innocent peoples and the innocent children looks like the river which stained with copious blood ,and the residues of the burned cadavers scattered between the damaged stones ,and the death mowed hundreds and thousands of the alive souls , the chests full of the rancor ,and the minds full of the revenges, the stones were on the point of speaking from the horrible pictures and the international nations were silent in spite of the terrible massacres ,and the revenge was from the second young man was strongly painful, he wraps his chest with the explosive belt to be looked like the nice muffler, he performs a strong and severe explosion between the innocent peoples |
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